Sękowska Alicja, Bogiel Tomasz
Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 9 M. Sklodowska-Curie Street, 85-094 Bydgoszcz, Poland.
Antibiotics (Basel). 2022 Jan 21;11(2):138. doi: 10.3390/antibiotics11020138.
Increasing antimicrobial resistance of Gram-negative rods is an important diagnostic, clinical and epidemiological problem of modern medicine. Therefore, it is important to detect multi-drug resistant strains as early on as possible. This study aimed to evaluate Eazyplex SuperBug CRE assay usefulness for beta-lactamase gene detection among Gram-negative rods, directly from urine samples and positive blood cultures. The Eazyplex SuperBug CRE assay is based on a loop-mediated isothermal amplification of genetic material and allows for the detection of a selection of genes encoding carbapenemases, KPC, NDM, VIM, OXA-48, OXA-181 and extended-spectrum beta-lactamases from the CTX-M-1 and CTX-M-9 groups. A total of 120 clinical specimens were included in the study. The test gave valid results for 58 (96.7%) urine samples and 57 (95.0%) positive blood cultures. ESBL and/or carbapenemase enzymes genes were detected in 56 (93.3%) urine and 55 (91.7%) blood samples, respectively. The Eazyplex SuperBug CRE assay can be used for a rapid detection of the genes encoding the most important resistance mechanisms to beta-lactams in Gram-negative rods also without the necessity of bacterial culture.
革兰氏阴性杆菌日益增强的抗菌耐药性是现代医学中一个重要的诊断、临床和流行病学问题。因此，尽早检测出多重耐药菌株至关重要。本研究旨在评估Eazyplex SuperBug CRE检测法直接从尿液样本和阳性血培养物中检测革兰氏阴性杆菌中β-内酰胺酶基因的有效性。Eazyplex SuperBug CRE检测法基于遗传物质的环介导等温扩增，可检测一系列编码碳青霉烯酶、KPC、NDM、VIM、OXA-48、OXA-181以及CTX-M-1和CTX-M-9组中的超广谱β-内酰胺酶的基因。本研究共纳入120份临床标本。该检测法对58份（96.7%）尿液样本和57份（95.0%）阳性血培养物给出了有效结果。分别在56份（93.3%）尿液样本和55份（91.7%）血样本中检测到ESBL和/或碳青霉烯酶基因。Eazyplex SuperBug CRE检测法可用于快速检测革兰氏阴性杆菌中编码对β-内酰胺最重要耐药机制的基因，且无需进行细菌培养。
