Tenover Fred C, Tickler Isabella A
Cepheid, Sunnyvale, CA 94089, USA.
Antibiotics (Basel). 2022 Feb 12;11(2):239. doi: 10.3390/antibiotics11020239.
The application of molecular detection methods for bacterial pathogens has dramatically improved the outcomes of septic patients, including those with methicillin-resistant (MRSA) infections. Molecular methods can be applied to a variety of clinical specimens including nasal swabs, growth in blood culture bottles, and wounds. While data show that the overall accuracy of molecular tests for MRSA is high, results can be confounded by the presence of multiple staphylococcal species in a specimen, insertions and deletions of DNA in and around the Staphylococcal Cassette Chromosome (SCC) element, and point mutations in . Herein, we explore the complexities of molecular approaches to MRSA detection and the instances where phenotypic methods should be pursued to resolve discrepancies between genotypic and phenotypic results.
用于检测细菌病原体的分子检测方法的应用显著改善了脓毒症患者的治疗结果，包括耐甲氧西林金黄色葡萄球菌（MRSA）感染患者。分子方法可应用于多种临床标本，包括鼻拭子、血培养瓶中的培养物以及伤口。虽然数据表明MRSA分子检测的总体准确性很高，但标本中多种葡萄球菌物种的存在、葡萄球菌盒式染色体（SCC）元件及其周围DNA的插入和缺失以及[此处原文缺失部分内容]中的点突变可能会混淆结果。在此，我们探讨了MRSA检测分子方法的复杂性，以及在哪些情况下应采用表型方法来解决基因型和表型结果之间的差异。
