Iqneibi Shahad, Nazzal Jamil, Owda Basma, Sultan Hala, Amoudi Runa, Amarin Justin Z, Al-Ghnimat Sura, Ahram Mamoun, Al-Hussaini Maysa
School of Medicine, The University of Jordan, Amman 11942, Jordan.
Office of Scientific Affairs and Research, King Hussein Cancer Center, Amman 11941, Jordan.
Brain Sci. 2022 Feb 17;12(2):282. doi: 10.3390/brainsci12020282.
p27 and p57 are tumor suppressors that are dysregulated in many cancers. We investigated the immunohistochemical expression of p27 and p57 in ependymoma, with a secondary emphasis on cyclin D1, nestin, and Ki-67. Sixty-five patients diagnosed with ependymoma were included. Clinical and tumoral data were retrieved, and the expression of p27, p57, cyclin D1, nestin, and Ki-67 was measured. Pearson's test was used to measure associations and the Kaplan-Meier method was used for survival analysis. p27 underexpression was significantly associated with pseudopalisading necrosis in tumors with foci of necrosis ( = 0.004). Cyclin D1 overexpression was associated with intracranial ( = 0.044), recurrent ( = 0.022) and grade 3 tumors ( = 0.016); nestin overexpression was associated with supratentorial ( = 0.025), mitotically active ( < 0.001), and grade 3 tumors ( = 0.004); Ki-67 overexpression was associated with supratentorial ( = 0.044) and grade 3 tumors ( < 0.001) and the 3 main features of anaplasia. None of the markers were intercorrelated or predictive of overall survival. In conclusion, p27 underexpression in tumors with foci of necrosis signals a pseudopalisading pattern. Cyclin D1, nestin, and Ki-67 are useful markers in ependymoma, but evidence-based cutoff values are required to standardize this interpretation.
p27和p57是肿瘤抑制因子，在许多癌症中表达失调。我们研究了p27和p57在室管膜瘤中的免疫组化表达，其次重点研究了细胞周期蛋白D1、巢蛋白和Ki-67。纳入了65例诊断为室管膜瘤的患者。收集临床和肿瘤数据，并检测p27、p57、细胞周期蛋白D1、巢蛋白和Ki-67的表达。采用Pearson检验测量相关性，采用Kaplan-Meier法进行生存分析。在有坏死灶的肿瘤中，p27低表达与假栅栏状坏死显著相关（P = 0.004）。细胞周期蛋白D1过表达与颅内肿瘤（P = 0.044）、复发性肿瘤（P = 0.022）和3级肿瘤（P = 0.016）相关；巢蛋白过表达与幕上肿瘤（P = 0.025）、有丝分裂活跃的肿瘤（P < 0.001）和3级肿瘤（P = 0.004）相关；Ki-67过表达与幕上肿瘤（P = 0.044）和3级肿瘤（P < 0.001）以及间变的3个主要特征相关。这些标志物均无相互关联或对总生存有预测作用。总之，有坏死灶的肿瘤中p27低表达提示假栅栏状模式。细胞周期蛋白D1、巢蛋白和Ki-67是室管膜瘤的有用标志物，但需要基于证据的临界值来规范这种解读。
