Ioachim Elli E, Katsanos Konstantinos H, Michael Michael C, Tsianos Epameinondas V, Agnantis Niki J
Department Pathology (Hepato-Gastroenterology Unit), Medical School, University of Ioannina, 451 10 Ioannina, Greece.
Int J Colorectal Dis. 2004 Jul;19(4):325-33. doi: 10.1007/s00384-003-0571-3. Epub 2004 Apr 2.
The expression patterns of cyclins D1 and E as well as cyclin-dependent kinase inhibitors p21/waf1 and p27/kip1 and their correlation with clinical parameters and other cell cycle regulators was investigated in inflammatory bowel disease (IBD).
These molecular markers were localized immunohistochemically using the monoclonal antibodies anti-cyclin D1 (DCS-6), anti-cyclin E (13A3), anti-p21 (4D10) and anti-p27 (1B4) in 70 patients with IBD, 30 patients with colorectal cancer and eight healthy subjects. Data were analyzed statistically using the software program.
Cyclin D1 expression was higher in both UC and CD compared with the healthy control group. In addition, CD cyclin D1 expression was higher compared with UC cases and colorectal carcinomas. Cyclin D1 expression was correlated with disease activity and cell proliferation in UC cases. A positive relationship of cyclin D1 with p27/kip1 in both UC and CD was detected. Cyclin E expression was higher in UC, CD and carcinomas compared with healthy control group and its expression correlated with proliferative activity in both UC and CD cases. p21/waf1 expression was higher in IBD cases compared with that of the control group, while a decreased p21/waf1 expression in the group of carcinomas was noted. This expression was correlated with disease activity in UC and the proliferative activity in both UC and CD. The expression of cyclins D1 and E as well as p21/waf1 was also correlated with the existence of dysplastic lesions. A lower p27/kip1 expression in the group of carcinomas compared with IBD cases and healthy controls was found.
The expression patterns of cyclin D1, cyclin E, p21/waf1 and p27/kip1 in IBD may indicate their contribution in epithelial cell turnover and their possible implication in IBD-related dysplasia-carcinoma.
研究细胞周期蛋白D1和E以及细胞周期蛋白依赖性激酶抑制剂p21/waf1和p27/kip1在炎症性肠病（IBD）中的表达模式，及其与临床参数和其他细胞周期调节因子的相关性。
使用抗细胞周期蛋白D1（DCS-6）、抗细胞周期蛋白E（13A3）、抗p21（4D10）和抗p27（1B4）单克隆抗体，通过免疫组织化学方法对70例IBD患者、30例结直肠癌患者和8名健康受试者的这些分子标志物进行定位。使用软件程序对数据进行统计学分析。
与健康对照组相比，UC和CD中细胞周期蛋白D1的表达均较高。此外，CD中细胞周期蛋白D1的表达高于UC病例和结直肠癌。在UC病例中，细胞周期蛋白D1的表达与疾病活动度和细胞增殖相关。在UC和CD中均检测到细胞周期蛋白D1与p27/kip1呈正相关。与健康对照组相比，UC、CD和癌组织中细胞周期蛋白E的表达均较高，且其表达与UC和CD病例中的增殖活性相关。IBD病例中p21/waf1的表达高于对照组，而癌组织组中p21/waf1的表达降低。该表达与UC中的疾病活动度以及UC和CD中的增殖活性相关。细胞周期蛋白D1和E以及p21/waf1的表达也与发育异常病变的存在相关。与IBD病例和健康对照组相比，癌组织组中p27/kip1的表达较低。
IBD中细胞周期蛋白D1、细胞周期蛋白E、p21/waf1和p27/kip1的表达模式可能表明它们在上皮细胞更新中的作用及其在IBD相关发育异常-癌中的可能意义。
