Araújo Natália Pereira da Silva, de Matos Natália Alves, Oliveira Michel, de Souza Ana Beatriz Farias, Castro Thalles de Freitas, Machado-Júnior Pedro Alves, de Souza Débora Maria Soares, Talvani André, Cangussú Sílvia Dantas, de Menezes Rodrigo Cunha Alvim, Bezerra Frank Silva
Laboratory of Experimental Pathophysiology, Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, Brazil.
Laboratory of Immunobiology of Inflammation, Department of Biological Sciences, Institute of Exact and Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, Brazil.
Antioxidants (Basel). 2022 Jan 18;11(2):181. doi: 10.3390/antiox11020181.
Chronic obstructive pulmonary disease (COPD) is the major cause of morbidity and mortality worldwide, and cigarette smoke is a key factor in the development of COPD. Thus, the development of effective therapies to prevent the advancement of COPD has become increasingly essential. We hypothesized that quercetin protects lungs in mice exposed to long-term cigarette smoke. Thirty-five C57BL/6 mice were exposed to cigarette smoke (12 cigarettes per day) for 60 days and pretreated with 10 mg/kg/day of quercetin via orogastric gavage. After the experimental protocol, the animals were euthanized and samples were collected for histopathological, antioxidant defense, oxidative stress and inflammatory analysis. The animals exposed to cigarette smoke showed an increase in respiratory rate and hematological parameters, cell influx into the airways, oxidative damage and inflammatory mediators, besides presenting with alterations in the pulmonary histoarchitecture. The animals receiving 10 mg/kg/day of quercetin that were exposed to cigarette smoke presented a reduction in cellular influx, less oxidative damage, reduction in cytokine levels, improvement in the histological pattern and improvement in pulmonary emphysema compared to the group that was only exposed to cigarette smoke. These results suggest that quercetin may be an agent in preventing pulmonary emphysema induced by cigarette smoke.
慢性阻塞性肺疾病（COPD）是全球发病和死亡的主要原因，而香烟烟雾是COPD发展的关键因素。因此，开发有效的疗法来预防COPD的进展变得越来越重要。我们假设槲皮素可以保护长期暴露于香烟烟雾中的小鼠的肺部。将35只C57BL/6小鼠暴露于香烟烟雾（每天12支香烟）中60天，并通过灌胃给予10mg/kg/天的槲皮素进行预处理。实验方案结束后，对动物实施安乐死并收集样本进行组织病理学、抗氧化防御、氧化应激和炎症分析。暴露于香烟烟雾的动物除了肺部组织结构发生改变外，还表现出呼吸频率和血液学参数增加、气道细胞流入、氧化损伤和炎症介质增加。与仅暴露于香烟烟雾的组相比，接受10mg/kg/天槲皮素且暴露于香烟烟雾的动物表现出细胞流入减少、氧化损伤减轻、细胞因子水平降低、组织学模式改善和肺气肿改善。这些结果表明，槲皮素可能是预防香烟烟雾诱导的肺气肿的一种药物。
