DeOliveira-Mello Laura, Vicente Isabel, Gonzalez-Nunez Veronica, Santos-Ledo Adrian, Velasco Almudena, Arévalo Rosario, Lara Juan M, Mack Andreas F
Institute of Neurosciences of Castilla and León, University of Salamanca, 37007 Salamanca, Spain.
Institute for Biomedical Research of Salamanca (IBSAL), University of Salamanca, 37007 Salamanca, Spain.
Biology (Basel). 2022 Feb 6;11(2):248. doi: 10.3390/biology11020248.
Doublecortin (DCX) is a microtubule associated protein, essential for correct central nervous system development and lamination in the mammalian cortex. It has been demonstrated to be expressed in developing-but not in mature-neurons. The teleost visual system is an ideal model to study mechanisms of adult neurogenesis due to its continuous life-long growth. Here, we report immunohistochemical, , and western blot analysis to detect the DCX protein in the visual system of teleost fish. We clearly determined the expression of DCX in newly generated cells in the retina of the cichlid fish , but not in the cyprinid fish . Here, we show that DCX is not associated with migrating cells but could be related to axonal growth. This work brings to light the high conservation of DCX sequences between different evolutionary groups, which make it an ideal marker for maturing neurons in various species. The results from different techniques corroborate the absence of DCX expression in zebrafish. In , DCX is very useful for identifying new neurons in the transition zone of the retina. In addition, this marker can be applied to follow axons from maturing neurons through the neural fiber layer, optic nerve head, and optic nerve.
双皮质素（DCX）是一种微管相关蛋白，对哺乳动物皮质中正确的中枢神经系统发育和分层至关重要。已证明它在发育中的神经元中表达，而不在成熟神经元中表达。硬骨鱼的视觉系统由于其终生持续生长，是研究成体神经发生机制的理想模型。在此，我们报告免疫组织化学、 以及蛋白质印迹分析，以检测硬骨鱼视觉系统中的DCX蛋白。我们明确确定了双皮质素在丽鱼科鱼类视网膜新生成细胞中的表达，但在鲤科鱼类中未检测到。在此，我们表明双皮质素与迁移细胞无关，但可能与轴突生长有关。这项工作揭示了不同进化群体之间双皮质素序列的高度保守性，这使其成为各种物种中成熟神经元的理想标记物。不同技术的结果证实斑马鱼中不存在双皮质素表达。在 中，双皮质素对于识别视网膜过渡区的新神经元非常有用。此外，该标记物可用于追踪成熟神经元的轴突穿过神经纤维层、视神经乳头和视神经。
