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丁香提取物用于珊瑚纤毛虫感染药物治疗的评估()

Evaluation of Clove Extract for Drug Therapy of Ciliate Infection in Coral ().

作者信息

Chu Tah-Wei, Cheng Chiu-Min, Cheng Yu-Rong, Dong Cheng-Di, Chuang Chih-Hung, Pan Chih-Hung, Sun Wei-Ting, Ding De-Sing

机构信息

Department and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology, Kaohsiung 811, Taiwan.

Department of Fisheries Production and Management, National Kaohsiung University of Science and Technology, No. 142, Haijhuan Rd., Nanzih District, Kaohsiung 811, Taiwan.

出版信息

Biology (Basel). 2022 Feb 10;11(2):280. doi: 10.3390/biology11020280.

Abstract

In recent years, ciliate infections have caused serious casualties to corals in the ocean. Infected corals die within a short period of time, which not only poses a threat to wild coral reefs, but also has a major impact on large scale aquaculture of coral. Clove is a kind of Chinese medicine with antifungal, antibacterial, antiviral, insecticidal, and other functions. Clove is a natural product. If it can be used in the treatment of coral ciliates, it will reduce this threat to the environment. The clove extract was diluted with sterile seawater to 500 ppm, 1500 ppm, 2500 ppm, 5000 ppm, 7500 ppm, and 10,000 ppm to carry out virulence test on ciliates. The results show that the LC value is 1500 ppm, which can cause the death of ciliates in 10 min without causing significant changes in SOD, CAT, chlorophyll a, and zooxanthellae. In addition, observation of tissue slices revealed that no ciliates and vacuum were found in the tissue after 10 min of medicated bathing. In summary, 1500 ppm of clove extract can be used for the treatment of coral ciliates.

摘要

近年来,纤毛虫感染给海洋中的珊瑚造成了严重伤亡。受感染的珊瑚在短时间内死亡,这不仅对野生珊瑚礁构成威胁,也对大规模珊瑚养殖产生重大影响。丁香是一种具有抗真菌、抗菌、抗病毒、杀虫等功能的中药。丁香是一种天然产物。如果它能用于治疗珊瑚纤毛虫,将减少对环境的这种威胁。将丁香提取物用无菌海水稀释至500 ppm、1500 ppm、2500 ppm、5000 ppm、7500 ppm和10000 ppm,对纤毛虫进行毒力试验。结果表明,LC值为1500 ppm,可在10分钟内导致纤毛虫死亡,且不会引起超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、叶绿素a和虫黄藻的显著变化。此外,组织切片观察显示,药浴10分钟后,组织中未发现纤毛虫和空泡。综上所述,1500 ppm的丁香提取物可用于治疗珊瑚纤毛虫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d831/8869591/5bd589436cc6/biology-11-00280-g001.jpg

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