Department of Microbiology, All India Institute of Medical Sciences, Bhopal 462020, Madhya Pradesh, India.
Infectious Diseases and Immunity in Global Health Program, Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada.
Genes (Basel). 2022 Jan 29;13(2):263. doi: 10.3390/genes13020263.
Uncontrolled transmission of (, MTB) drug resistant strains is a challenge to control efforts of the global tuberculosis program. Due to increasing multi-drug resistant (MDR) cases in Arunachal Pradesh, a northeastern state of India, the tracking and tracing of these resistant MTB strains is crucial for infection control and spread of drug resistance. This study aims to correlate the phenotypic DST, genomic DST (gDST) and phylogenetic analysis of MDR-MTB strains in the region. Of the total 200 samples 22 (11%) patients suspected of MDR-TB and 160 (80%) previously treated MDR-TB cases, 125 (62.5%) were identified as MTB. MGIT-960 SIRE DST detected 71/125 (56.8%) isolates as MDR/RR-MTB of which 22 (30.9%) were detected resistant to second-line drugs. Whole-genome sequencing of 65 isolates and their gDST found Ser315Thr mutation in (35/45; 77.8%) and 531Leu mutation in (21/41; 51.2%) associated with drug resistance. SNP barcoding categorized the dataset with Lineage2 (41; 63.1%) being predominant followed by Lineage3 (10; 15.4%), Lineage1 (8; 12.3%) and Lineage4 (6; 9.2%) respectively. Phylogenetic assignment by cgMLST gave insights of two Beijing sub-lineages viz; 2.2.1 (SNP difference < 19) and 2.2.1.2 (SNP difference < 9) associated with recent ongoing transmission in Arunachal Pradesh. This study provides insights in identifying two virulent Beijing sub-lineages (sub-lineage 2.2.1 and 2.2.1.2) with ongoing transmission of TB drug resistance in Arunachal Pradesh.
（MTB）耐药菌株的失控传播是全球结核病规划控制工作面临的挑战。由于印度东北部的阿鲁纳恰尔邦（Arunachal Pradesh）耐多药（MDR）病例不断增加，对这些耐药 MTB 菌株的跟踪和溯源对于感染控制和耐药传播至关重要。本研究旨在对该地区 MDR-MTB 菌株的表型 DST、基因组 DST（gDST）和系统发育分析进行相关性研究。在总共 200 个样本中，有 22 名（11%）疑似 MDR-TB 患者和 160 名（80%）既往治疗过的 MDR-TB 患者，125 名（62.5%）被鉴定为 MTB。MGIT-960 SIRE DST 检测到 71/125（56.8%）分离株为 MDR/RR-MTB，其中 22 株（30.9%）对二线药物耐药。对 65 株分离株进行全基因组测序及其 gDST 检测发现，在（35/45；77.8%）和（21/41；51.2%）中发现 Ser315Thr 突变与耐药性相关。SNP 条码分类数据集以 Lineage2（41；63.1%）为主，其次是 Lineage3（10；15.4%）、Lineage1（8；12.3%）和 Lineage4（6；9.2%）。cgMLST 系统发育赋值揭示了两个北京亚系，即 2.2.1（SNP 差异<19）和 2.2.1.2（SNP 差异<9），与阿鲁纳恰尔邦目前正在发生的结核病耐药传播有关。本研究提供了在阿鲁纳恰尔邦发现两个具有高毒力的北京亚系（亚系 2.2.1 和 2.2.1.2），以及正在发生的结核病耐药传播的见解。
