鉴定CesA 亚家族和 GhMCesA35 在 L. 中的功能分析

Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in L.

机构信息

Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.

出版信息

Genes (Basel). 2022 Feb 1;13(2):292. doi: 10.3390/genes13020292.

The cellulose synthase genes control the biosynthesis of cellulose in plants. Nonetheless, the gene family members of CesA have not been identified in the newly assembled genome of (AD1, HEBAU_NDM8). We identified 38 CesA genes in (NDM8) and found that the protein sequence of GhMCesA35 is 100% identical to CelA1 in a previous study. It is already known that CelA1 is involved in cellulose biosynthesis in vitro. However, the function of this gene in vivo has not been validated. In this study, we verified the function of GhMCesA35 in vivo based on overexpressed . In addition, we found that it interacted with GhCesA7 through the yeast two-hybrid assay. This study provides new insights for studying the biological functions of CesA genes in , thereby improving cotton fiber quality and yield.

纤维素合酶基因控制植物中纤维素的生物合成。然而，在新组装的（AD1、HEBAU_NDM8）基因组中尚未鉴定出 CesA 的基因家族成员。我们在（NDM8）中鉴定出 38 个 CesA 基因，并发现 GhMCesA35 的蛋白序列与之前研究中的 CelA1 完全相同。已知 CelA1 参与体外纤维素的生物合成。然而，该基因在体内的功能尚未得到验证。在这项研究中，我们基于过表达（GhMCesA35）在体内验证了 GhMCesA35 的功能。此外，我们通过酵母双杂交试验发现它与 GhCesA7 相互作用。本研究为研究 CesA 基因在（AD1、HEBAU_NDM8）中的生物学功能提供了新的见解，从而提高了棉花纤维的质量和产量。