工程化生产植物储存蛋白的真菌。
Engineered Fungus Producing Plant Storage Proteins.
作者信息
Balabanova Larissa, Seitkalieva Aleksandra, Yugay Yulia, Rusapetova Tatiana, Slepchenko Lubov, Podvolotskaya Anna, Yatsunskaya Margarita, Vasyutkina Elena, Son Oksana, Tekutyeva Liudmila, Shkryl Yury
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Prospect 100-letya Vladivostoka 159, 690022 Vladivostok, Russia.
Federal Scientific Centre of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Prospect 100-letya Vladivostoka 159, 690022 Vladivostok, Russia.
出版信息
J Fungi (Basel). 2022 Jan 26;8(2):119. doi: 10.3390/jof8020119.
An efficient -mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for the multiple heterologous protein production in filamentous fungus F-859 (formerly F-859). The engineered fungus was able to produce plant storage proteins of (α-zein Z19) and (albumin A1) to enrich fungal biomass by valuable nutritional proteins and improved amino acid content. The mRNA levels of and genes were significantly dependent on their driving promoters: the promoter of tryptophan synthase (P) was more efficient to express , while the promoter of translation elongation factor (P) provided much higher levels of transcript abundance. In general, the total recombinant proteins and amino acid contents were higher in the P-containing clones. This work describes a new strategy to improve mycoprotein nutritive value by overexpression of plant storage proteins.
基于植物双元载体pPZP-RCS2进行了高效介导的遗传转化，用于丝状真菌F-859（原F-859）中多种异源蛋白的生产。工程化真菌能够产生植物储存蛋白（α-玉米醇溶蛋白Z19）和（白蛋白A1），通过有价值的营养蛋白和改善的氨基酸含量来丰富真菌生物量。和基因的mRNA水平显著依赖于它们的驱动启动子：色氨酸合酶启动子（P）在表达方面更有效，而翻译延伸因子启动子（P）提供了更高水平的转录本丰度。一般来说，含P的克隆中总重组蛋白和氨基酸含量更高。这项工作描述了一种通过过表达植物储存蛋白来提高真菌蛋白营养价值的新策略。
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