Department of General Surgery, Faculty of General Medicine, "Coltea" Hospital, Carol Davila University, 020021 Bucharest, Romania.
Department of General Surgery, Medical Faculty, Kocaeli University, Kocaeli 41380, Turkey.
Int J Environ Res Public Health. 2022 Feb 14;19(4):2138. doi: 10.3390/ijerph19042138.
Obesity is associated with endothelial dysfunction and this relationship is probably mediated in part by inflammation. The current study evaluated the effects of etanercept, a tumor necrosis factor-alpha (TNF-α) inhibitor, on endothelial and vascular reactivity, endothelial nitric oxide synthase (eNOS) immunoreactivity, and serum and aortic concentrations of TNF-α in a diet-induced rat model. Male weanling Wistar rats were exposed to a standard diet and cafeteria diet (CD) for 12 weeks and etanercept was administered during CD treatment. Isolated aortas of the rats were used for isometric tension recording. Carbachol-induced relaxant responses were impaired in CD-fed rats, while etanercept treatment improved these endothelium-dependent relaxations. No significant change was observed in papaverine- and sodium nitroprusside (SNP)-induced relaxant responses. eNOS expression decreased in CD-fed rats, but no change was observed between etanercept-treated CD-fed rats and control rats. CD significantly increased both the serum and the aortic levels of TNF-α, while etanercept treatment suppressed these elevated levels. CD resulted in a significant increase in the body weight of the rats. Etanercept-treated (ETA) CD-fed rats gained less weight than both CD-fed and control rats.
肥胖与血管内皮功能障碍有关，这种关系可能部分通过炎症介导。本研究评估了肿瘤坏死因子-α（TNF-α）抑制剂依那西普对饮食诱导的大鼠模型内皮和血管反应性、内皮型一氧化氮合酶（eNOS）免疫反应性以及血清和主动脉 TNF-α浓度的影响。雄性断乳 Wistar 大鼠暴露于标准饮食和自助餐厅饮食（CD）12 周，并在 CD 治疗期间给予依那西普。大鼠的离体主动脉用于等长张力记录。在 CD 喂养的大鼠中，卡巴胆碱诱导的舒张反应受损，而依那西普治疗改善了这些内皮依赖性舒张反应。罂粟碱和硝普钠（SNP）诱导的舒张反应没有观察到显著变化。CD 喂养的大鼠中 eNOS 表达减少，但依那西普治疗的 CD 喂养大鼠与对照组大鼠之间没有观察到变化。CD 显著增加了血清和主动脉 TNF-α 的水平，而依那西普治疗抑制了这些升高的水平。CD 导致大鼠体重显著增加。依那西普治疗（ETA）CD 喂养的大鼠比 CD 喂养的大鼠和对照组大鼠体重增加更少。
