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[检测患者粪便接种物裂解物中宋内痢疾病原体抗原的血清学方法]

[Serological method of detecting the antigens of the causative agent of Sonne dysentery in lysates of the fecal inoculates from patients].

作者信息

Seppi I V, Levi M I, Stepanova S L, Mel'nik E G, Amirkhanova A V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 May(5):31-6.

PMID:352065
Abstract

Serological method of detection of Sh. sonnei antigens in the lysates of the patients, fecal cultures is suggested and approved. In the majority of cases of the results of bacteriological and serological methods of study of the feces coincided. Data confirming the specificity of the antibody neutralization test (ANT) in Sonne dysentery are presented. In connection with detection of the screening action of the Vi-antigen of typhoid bacilli there were elaborated additional methods for verifying the specificity of the ANT results. It is recommended to keep agar plates after selection of suspicious colonies during the bacteriological test; the lysate of the microbial crop should be additionally subjected to the ANT, this considerably increasing the percentage of laboratory confirmations of dysentery caused by Sh. sonnei.

摘要

建议并批准了在患者裂解物中检测宋内志贺氏菌抗原的血清学方法以及粪便培养法。在大多数情况下,粪便细菌学和血清学研究方法的结果是一致的。文中给出了证实抗体中和试验(ANT)在宋内氏痢疾中具有特异性的数据。鉴于检测到伤寒杆菌Vi抗原的筛选作用,还制定了额外的方法来验证ANT结果的特异性。建议在细菌学检测中挑选可疑菌落之后保留琼脂平板;微生物培养物的裂解物应额外进行ANT检测,这大大提高了由宋内志贺氏菌引起的痢疾的实验室确诊率。

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