Zhang Peiwen, Zhou You, Qin Deqiang, Chen Jianjun, Zhang Zhixiang
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Mid-Florida Research and Education Center, Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, University of Florida, Apopka, FL 32703, USA.
Metabolites. 2022 Feb 8;12(2):158. doi: 10.3390/metabo12020158.
Biological control is a key component of integrated pest management (IPM). To suppress pests in a certain threshold, chemical control is used in combination with biological and other control methods. An essential premise for using pesticides in IPM is to ascertain their compatibility with beneficial insects. (Neuroptera: Chrysopidae) is an important predator of various pests and used for pest management. This study was intended to analyze metabolic changes in larvae after feeding on azadirachtin-treated (Lepidoptera, Plutellidae) larvae through a non-targeted LC-MS (Liquid chromatography-mass spectrometry) based metabolomics analysis. Results showed that larvae did not die after consuming larvae treated with azadirachtin. However, their pupation and eclosion were adversely affected, resulting in an impairment in the completion of their life cycle. Feeding larvae with azadirachtin-treated larvae affected over 10,000 metabolites across more than 20 pathways, including the metabolism of amino acids, carbohydrates, lipid, cofactors, and vitamins in larvae, of which changes in amnio acid metabolism were particularly pronounced. A working model was proposed to illustrate differential changes in 20 metabolites related to some amino acid metabolisms. Among them, 15 were markedly reduced and only five were elevated. Our results suggest that azadirachtin application may not be exclusively compatible with the use of the predator for control of . It is recommended that the compatibility should be evaluated not only based on the survival of the predatory insects but also by the metabolic changes and the resultant detrimental effects on their development.
生物防治是综合虫害管理（IPM）的关键组成部分。为了将害虫数量控制在一定阈值内，化学防治与生物防治及其他防治方法联合使用。在IPM中使用杀虫剂的一个基本前提是确定它们与益虫的兼容性。草蛉（脉翅目：草蛉科）是多种害虫的重要捕食者，可用于虫害管理。本研究旨在通过基于非靶向液相色谱 - 质谱联用（LC-MS）的代谢组学分析，分析草蛉幼虫取食经印楝素处理的小菜蛾（鳞翅目：菜蛾科）幼虫后的代谢变化。结果表明，草蛉幼虫取食经印楝素处理的小菜蛾幼虫后并未死亡。然而，它们的化蛹和羽化受到不利影响，导致其生命周期的完成受到损害。用经印楝素处理的小菜蛾幼虫喂食草蛉幼虫影响了20多条途径中的10000多种代谢物，包括草蛉幼虫体内氨基酸、碳水化合物、脂质、辅因子和维生素的代谢，其中氨基酸代谢的变化尤为明显。提出了一个工作模型来说明与某些氨基酸代谢相关的20种代谢物的差异变化。其中，15种显著减少，只有5种升高。我们的结果表明，印楝素的应用可能不完全与使用捕食性天敌草蛉来控制小菜蛾兼容。建议不仅应根据捕食性昆虫的存活情况，还应根据代谢变化及其对发育产生的有害影响来评估兼容性。
