Basic and Experimental Nutrition Department, Josué de Castro Nutrition Institute, Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, 393, Rio de Janeiro 21941-590, Brazil.
Institute of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Rio de Janeiro 21941-909, Brazil.
Molecules. 2022 Feb 10;27(4):1194. doi: 10.3390/molecules27041194.
Phenolic compounds (PCs) present in foods are associated with a decreased risk of developing inflammatory diseases. The aim of this study was to extract and characterize PCs from craft beer powder and evaluate their potential benefits in an experimental model of inflammatory bowel disease (IBD). PCs were extracted and quantified from pure beer samples. BALB/c mice received either the beer phenolic extract (BPE) or beer powder fortified with phenolic extract (BPFPE) of PCs daily for 20 days by gavage. Colon samples were collected for histopathological and immunohistochemical analyses. Dextran sodium sulfate (DSS)-induced mice lost more weight, had reduced colon length, and developed more inflammatory changes compared with DSS-induced mice treated with either BPE or BPFPE. In addition, in DSS-induced mice, the densities of CD4- and CD11b-positive cells, apoptotic rates, and activation of NF-κB and p-ERK1/2 MAPK intracellular signaling pathways were higher in those treated with BPE and BPFPE than in those not treated. Pretreatment with the phenolic extract and BPFPE remarkably attenuated DSS-induced colitis. The protective effect of PCs supports further investigation and development of therapies for human IBD.
酚类化合物（PCs）存在于食物中，与降低发生炎症性疾病的风险有关。本研究旨在从精酿啤酒粉中提取和鉴定 PCs，并在炎症性肠病（IBD）的实验模型中评估其潜在益处。从纯啤酒样品中提取和定量 PCs。BALB/c 小鼠通过灌胃每天接受啤酒酚提取物（BPE）或富含 PC 的啤酒粉（BPFPE）治疗 20 天。收集结肠样本进行组织病理学和免疫组织化学分析。与用 BPE 或 BPFPE 治疗的 DSS 诱导的小鼠相比，用 DSS 诱导的小鼠体重减轻更多，结肠长度缩短，炎症变化更多。此外，在 DSS 诱导的小鼠中，与未处理的小鼠相比，用 BPE 和 BPFPE 处理的小鼠中 CD4-和 CD11b-阳性细胞的密度、细胞凋亡率以及 NF-κB 和 p-ERK1/2 MAPK 细胞内信号通路的激活更高。用酚提取物和 BPFPE 预处理可显著减轻 DSS 诱导的结肠炎。PCs 的保护作用支持进一步研究和开发人类 IBD 的治疗方法。
