Programa Regional en Ciencias Veterinarias Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia 40101, Costa Rica.
Laboratorio de Fitoquímica, Escuela de Química, Universidad Nacional, Heredia 40101, Costa Rica.
Molecules. 2022 Feb 16;27(4):1348. doi: 10.3390/molecules27041348.
Vector-borne diseases, such as those transmitted by , are a constant threat to inhabitants of tropical regions of the planet. Synthetic chemicals are commonly used as a strategy to control them; however, these products are known to persist in ecosystems and drive the appearance of resistance genes in arthropod vectors. Thus, the use of natural products has emerged as an environmentally friendly alternative in integrated vector control strategies. The present bioguided study investigated the larvicidal potential of extracts, fractionated using thin-layer and open-column chromatography, because this species has been shown to exert larvicidal effects on the genus . The objective of this study was to evaluate the nonvolatile components in ethanolic extract of stems as a potential natural larvicidal, and coumarins, such as 7-hydroxy-6-methoxychromen-2-one (scopoletin) and 7-hydroxychromen-2-one (umbelliferone), were identified as major compounds; however, they were not shown to be responsible for the larvicidal activity. Based on the results of the larvicidal action tests, these coumarins are not directly responsible for the larvicidal activity, but this activity might be attributed to a synergistic effect of all the compounds present in the most active secondary fraction, called F.DCM, which had an LC value of 30.608 mg/L. This type of study has yet not been conducted in the region; therefore, it is an important contribution to recognizing a natural and easy-to-cultivate source of vector control, such .
虫媒病，如由 传播的疾病，是地球上热带地区居民的持续威胁。合成化学品通常被用作控制它们的策略；然而，这些产品已知会在生态系统中持续存在，并促使节肢动物传播媒介中出现抗性基因。因此，天然产物的使用已成为综合病媒控制策略中一种环保的替代方法。本生物导向研究调查了 提取物的杀幼虫潜力，提取物使用薄层和开管色谱法进行了分离，因为已经证明该物种对 属具有杀幼虫作用。本研究的目的是评估 茎的乙醇提取物中的非挥发性成分作为潜在的天然杀幼虫剂，香豆素，如 7-羟基-6-甲氧基色原酮-2-酮（东莨菪内酯）和 7-羟基色原酮-2-酮（伞形酮），被鉴定为主要化合物；然而，它们并未显示出具有杀幼虫活性。根据杀幼虫作用测试的结果，这些香豆素并非直接负责杀幼虫活性，但这种活性可能归因于最活跃的次级部分（称为 F.DCM）中存在的所有化合物的协同作用，该部分的 LC 值为 30.608 mg/L。该地区尚未进行此类研究；因此，这是对识别天然且易于培养的病媒控制来源的重要贡献，例如 。
