Department of Microbiology, School of Science and Technology, PMB 4005, Babcock University, Ilisan-Remo, Ogun State, Nigeria.
Department of Botany, Faculty of Science, University of Ibadan, Ibadan, Oyo State, Nigeria.
Mol Biol Rep. 2021 Jan;48(1):41-55. doi: 10.1007/s11033-020-06119-6. Epub 2021 Jan 17.
Mosquito-transmitted diseases like zika, dengue, chikungunya, and yellow fever are known to affect human health worldwide. Numerous synthetic insecticides have been used as vector control for these diseases, but there is the challenge of environmental toxicity and vector resistance. This study investigated the medicinal and insecticidal potential of Lentinus squarrosulus against Aedes aegypti. The fruiting bodies were identified morphologically as well as using internal transcribed spacer (ITS) sequences for its molecular characterization. Genomic deoxyribonucleic acid (DNA) yield was confirmed with NanoDrop Spectrophotometer ND-1000 and amplified with ITSl and ITS4 primers. The amplicons were sequenced and the National Center for Biotechnology Information (NCBI) database identified the nucleotides. Its ethanol extract was subjected to phytochemical screening and gas chromatography mass spectrometry (GC-MS) analysis and tested against the pupa and fourth instar larva of Aedes aegypti with percentage mortality monitored. The Macrofungus was identified morphologically and confirmed with molecular characterization as Lentinus squarrosulus (LS). The gene sequence was deposited in GenBank (Accession number MK629662.1). GC-MS analysis showed that its ethanol extract has 25 bioactive compounds with 9,12-Octadecadienoic acid, ethyl ester having the highest percentage of 43.32% as well as methyl-2-oxo-1-pyrrolidine acetate and 17-octadecynoic acid having the lowest percentage (0.09%). The macrofungus contained varied concentrations of phytochemicals including phenols (159 mg/g GAE), tannins (1.6 mg/g TAE), and flavonoids (31.4 mg/g QE). The ethanol extract had significant potent effects on Aedes aegypti larva and pupa which could be due to the occurrence and abundance of 9,12-octadecadienoic acid in LS. The LC of the extract for larvicidal and pupicidal activities were 2.95 mg/mL and 3.55 mg/mL, respectively, while its LC were 6.31 mg/mL and 5.75 mg/mL respectively. Lentinus squarrosulus had insecticidal effects against the Aedes aegypti larva and pupa and possessed great potential as a source of alternative medicine and eco-friendly insecticides.
蚊媒传播的疾病，如 Zika、登革热、基孔肯雅热和黄热病，已知会影响全球人类健康。已经使用了许多合成杀虫剂来控制这些疾病的传播，但存在环境毒性和媒介抗药性的挑战。本研究调查了香菇对埃及伊蚊的药用和杀虫潜力。子实体通过形态学以及内部转录间隔区 (ITS) 序列进行了分子鉴定。使用 NanoDrop Spectrophotometer ND-1000 确认基因组脱氧核糖核酸 (DNA) 产量，并使用 ITS1 和 ITS4 引物进行扩增。对扩增子进行测序，并使用国家生物技术信息中心 (NCBI) 数据库识别核苷酸。其乙醇提取物进行了植物化学筛选和气相色谱质谱 (GC-MS) 分析，并对埃及伊蚊的蛹和第四龄幼虫进行了测试，监测死亡率。大型真菌通过形态学鉴定，并通过分子鉴定确认为香菇 (LS)。基因序列已存入 GenBank（注册号 MK629662.1）。GC-MS 分析表明，其乙醇提取物含有 25 种具有生物活性的化合物，其中 9,12-十八碳二烯酸乙酯的含量最高，为 43.32%，其次是甲基-2-氧代-1-吡咯烷乙酸酯和 17-十八碳炔酸，含量最低（0.09%）。大型真菌含有不同浓度的植物化学物质，包括酚类（159mg/g GAE）、单宁（1.6mg/g TAE）和类黄酮（31.4mg/g QE）。乙醇提取物对埃及伊蚊幼虫和蛹具有显著的有效作用，这可能是由于 LS 中 9,12-十八碳二烯酸的存在和丰度所致。提取物的幼虫和蛹的 LC 分别为 2.95mg/mL 和 3.55mg/mL，而其 LC 分别为 6.31mg/mL 和 5.75mg/mL。香菇对埃及伊蚊幼虫和蛹具有杀虫作用，具有作为替代医学和环保型杀虫剂的巨大潜力。
