Biomedical Research Institute (BIOMED), Hasselt University (UHasselt), Diepenbeek, Belgium.
Pole of Pharmacology and Therapeutics, Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain (UCLouvain), Brussels, Belgium.
J Cereb Blood Flow Metab. 2022 Jun;42(6):979-996. doi: 10.1177/0271678X221084410. Epub 2022 Feb 25.
Extremely low frequency electromagnetic stimulation (ELF-EMS) has been considered as a neuroprotective therapy for ischemic stroke based on its capacity to induce nitric oxide (NO) signaling. Here, we examined whether ELF-EMS reduces ischemic stroke volume by stimulating cerebral collateral perfusion. Moreover, the pathway responsible for ELF-EMS-induced NO production was investigated. ELF-EMS diminished infarct growth following experimental stroke in collateral-rich C57BL/6 mice, but not in collateral-scarce BALB/c mice, suggesting that decreased lesion sizes after ELF-EMS results from improved collateral blood flow. analysis demonstrated that ELF-EMS increased endothelial NO levels by stimulating the Akt-/eNOS pathway. Furthermore, ELF-EMS augmented perfusion in the hind limb of healthy mice, which was mediated by enhanced Akt-/eNOS signaling. In healthy C57BL/6 mouse brains, ELF-EMS treatment increased cerebral blood flow in a NOS-dependent manner, whereas no improvement in cerebrovascular perfusion was observed in collateral-sparse BALB/c mice. In addition, ELF-EMS enhanced cerebral blood flow in both the contra- and ipsilateral hemispheres of C57BL/6 mice subjected to experimental ischemic stroke. In conclusion, we showed that ELF-EMS enhances (cerebro)vascular perfusion by stimulating NO production, indicating that ELF-EMS could be an attractive therapeutic strategy for acute ischemic stroke by improving cerebral collateral blood flow.
极低频电磁场刺激 (ELF-EMS) 因其能够诱导一氧化氮 (NO) 信号而被认为是一种缺血性中风的神经保护治疗方法。在这里，我们研究了 ELF-EMS 是否通过刺激脑侧支灌注来减少缺血性中风的体积。此外，还研究了 ELF-EMS 诱导 NO 产生的途径。ELF-EMS 减少了侧支丰富的 C57BL/6 小鼠实验性中风后的梗塞生长，但在侧支稀少的 BALB/c 小鼠中没有减少，这表明 ELF-EMS 后病变体积的减少是由于侧支血流的改善所致。 分析表明，ELF-EMS 通过刺激 Akt-/eNOS 途径增加内皮细胞 NO 水平。此外，ELF-EMS 增强了健康小鼠后肢的灌注，这是通过增强 Akt-/eNOS 信号介导的。在健康的 C57BL/6 小鼠大脑中，ELF-EMS 以 NOS 依赖的方式增加脑血流，而在侧支稀少的 BALB/c 小鼠中则没有观察到脑血管灌注的改善。此外，ELF-EMS 增强了 C57BL/6 小鼠实验性缺血性中风后对侧和同侧半球的脑血流。总之，我们表明 ELF-EMS 通过刺激 NO 产生来增强 (脑) 血管灌注，表明 ELF-EMS 通过改善脑侧支血流可能成为急性缺血性中风的一种有吸引力的治疗策略。
