Li Qi, Li Jing, Li Chong, Wu Xing, Si Sujin, Yang Pengkun, Li Wenting, Han Ruili, Li Guoxi, Liu Xiaojun, Kang Xiangtao, Tian Yadong
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.
College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
Anim Biotechnol. 2023 Nov;34(4):1342-1353. doi: 10.1080/10495398.2021.2024217. Epub 2022 Feb 24.
Long non-coding RNAs (lncRNAs) play important roles in transcriptional and post-transcriptional regulation. LncRNAs, which are defined as non-coding RNAs more than 200 bp in length, are involved in key biological processes, such as cell proliferation and differentiation, epigenetic regulation, and gene transcriptional translation. Recent studies have shown that lncRNAs also play major regulatory roles in the reproduction of mammals. However, knowledge of the roles of lncRNAs in the chicken ovary lacking. In this study, we performed RNA-seq analyses of ovarian tissue from Hy-Line brown laying hens at four physiological stages [15, 20, 30, and 68 weeks of age (W)]. We identified 657 lncRNA transcripts that were differentially expressed during ovarian development, the number of down-regulated lncRNAs was higher than the number of up-regulated lncRNAs during development. We predicted the cis and trans target genes of the DE lncRNAs and constructed a lncRNA-mRNA interaction network, which indicated that the DE genes (DEGs) and the target genes of the DE lncRNAs are mainly involved in signaling pathways associated with ovarian development, including oocyte meiosis, calcium signaling pathways, ECM-receptor interactions, and ribosome and focal adhesion. Overall, we found that twelve lncRNAs were strongly involved in ovarian development: LNC_013443, LNC_001029, LNC_005713, LNC_016762, ENSGALT00000101857, LNC_003913, LNC_013692, LNC_012219, LNC_004140, ENSGALT00000096941, LNC_009356, and ENSGALT00000098716. In summary, our study utilized RNA-seq analysis of hen ovaries to explore key lncRNAs involved in ovarian development and function. Furthermore, the comprehensive analysis identified the target genes of these lncRNAs providing a better understanding of the mechanisms underlying ovarian development in hens and a theoretical basis for further research.
长链非编码RNA（lncRNAs）在转录和转录后调控中发挥着重要作用。lncRNAs被定义为长度超过200bp的非编码RNA，参与细胞增殖与分化、表观遗传调控以及基因转录翻译等关键生物学过程。最近的研究表明，lncRNAs在哺乳动物生殖中也发挥着主要调控作用。然而，lncRNAs在鸡卵巢中的作用尚不清楚。在本研究中，我们对海兰褐蛋鸡四个生理阶段（15、20、30和68周龄）的卵巢组织进行了RNA测序分析。我们鉴定出657个在卵巢发育过程中差异表达的lncRNA转录本，发育过程中下调的lncRNAs数量高于上调的lncRNAs数量。我们预测了差异表达lncRNAs的顺式和反式靶基因，并构建了lncRNA- mRNA相互作用网络，这表明差异表达基因（DEGs）和差异表达lncRNAs的靶基因主要参与与卵巢发育相关的信号通路，包括卵母细胞减数分裂、钙信号通路、细胞外基质-受体相互作用以及核糖体和粘着斑。总体而言，我们发现十二个lncRNAs与卵巢发育密切相关：LNC_013443、LNC_001029、LNC_005713、LNC_016762、ENSGALT00000101857、LNC_003913、LNC_013692、LNC_012219、LNC_004140、ENSGALT00000096941、LNC_009356和ENSGALT00000098716。总之，我们的研究利用母鸡卵巢的RNA测序分析来探索参与卵巢发育和功能的关键lncRNAs。此外，综合分析确定了这些lncRNAs的靶基因，为更好地理解母鸡卵巢发育的潜在机制提供了依据，并为进一步研究奠定了理论基础。
