[苏联用于细胞培养的微载体研究]
[Investigation of Soviet microcarriers for cell cultivation].
作者信息
Zhesterev V I, Sergeev V A, Khizhinskaia V P, Grudina N V, Makarova S B
出版信息
Tsitologiia. 1986 Apr;28(4):465-9.
PMID:3521014
Abstract
Home made and foreign preparations (6 patterns in each) were tested as microcarriers for cultivation of anchorage-dependent cells in the medium supplemented with 0.25% enzymatic hydrolysate of muscles and 10-20% animal serum. The best results were obtained with microcarriers DEAE-2.5, Cytodex-1, and DEAE-cellulose. Using home microcarriers DEAE-2.5, continuous swine embryo kidney cells and primary chick embryo cells yielded, respectively, 9-11 and 3-4 times increase.
摘要
测试了国产和国外制剂（各6种）作为微载体，用于在添加了0.25%肌肉酶解物和10 - 20%动物血清的培养基中培养贴壁依赖性细胞。使用DEAE - 2.5、Cytodex - 1和DEAE - 纤维素微载体获得了最佳结果。使用国产微载体DEAE - 2.5，猪胚胎肾连续细胞和鸡胚胎原代细胞的产量分别提高了9 - 11倍和3 - 4倍。
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