Kotler M, Reuveny S, Mizrahi A, Shahar A
Dev Biol Stand. 1985;60:255-61.
Transformed embryonic avian and mammalian cells, as well as cells from established cell lines grow as a monolayer on DEAE-cellulose based microcarriers (MC): the DE-52 and DE-53 MC. Normal, non-transformed, embryonic avian and mammalian cells do not grow on DE-52 MC (having an exchange capacity of 1 meq/g dry material) but grow well on DE-53 MC (having an exchange capacity of 2 meq/g dry material). On DE-53 MC embryonic non-transformed cells grow in multilayers, while embryonic viral-transformed cells and cells from established cell lines grow in a monolayer form. Possible explanations for the differences in cell growth on various MC and variation in the mode of growth in monolayer vs multilayer are discussed. These differences may serve as a valuable tool for separation and distinction between normal and transformed cells. In addition, the described novel MC culturing system provides a support for tridimensional growth on which cell growth mimics the in vivo growth conditions. Therefore, this system is suitable for the study of cell recognition, cell to cell connection and cell orientation.
转化的胚胎禽细胞和哺乳动物细胞，以及来自已建立细胞系的细胞，能在基于二乙氨基乙基纤维素（DEAE - 纤维素）的微载体（MC）上形成单层生长，即DE - 52和DE - 53微载体。正常的、未转化的胚胎禽细胞和哺乳动物细胞不在DE - 52微载体（干物质交换容量为1 meq/g）上生长，但能在DE - 53微载体（干物质交换容量为2 meq/g）上良好生长。在DE - 53微载体上，未转化的胚胎细胞呈多层生长，而胚胎病毒转化细胞和来自已建立细胞系的细胞则呈单层生长。文中讨论了不同微载体上细胞生长差异以及单层生长与多层生长模式变化的可能原因。这些差异可能成为区分正常细胞和转化细胞的有价值工具。此外，所描述的新型微载体培养系统为三维生长提供了支持，细胞生长在此系统中模拟体内生长条件。因此，该系统适用于细胞识别、细胞间连接和细胞定向的研究。
