Sachs' Children and Youth Hospital, Dept of Pediatrics, Södersjukhuset, Stockholm, Sweden
Dept of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm, Sweden.
Eur Respir J. 2022 Sep 29;60(3). doi: 10.1183/13993003.02531-2021. Print 2022 Sep.
Bronchopulmonary dysplasia (BPD) in preterm-born infants is a risk factor for chronic airway obstruction in adulthood. Cytotoxic T-cells are implicated in COPD, but their involvement in BPD is not known.
To characterise the distribution of airway T-cell subsets in adults with a history of BPD.
Young adults with former BPD (n=22; median age 19.6 years), age-matched adults born preterm (n=22), patients with allergic asthma born at term (n=22) and healthy control subjects born at term (n=24) underwent bronchoalveolar lavage (BAL). T-cell subsets in BAL were analysed using flow cytometry.
The total number of cells and the differential cell counts in BAL were similar among the study groups. The percentage of CD3CD8 T-cells was higher (p=0.005) and the proportion of CD3CD4 T-cells was reduced (p=0.01) in the BPD group, resulting in a lower CD4/CD8 ratio (p=0.007) compared to the healthy controls (median 2.2 5.3). In BPD and preterm-born study subjects, both CD3CD4 T-cells (r=0.38, p=0.03) and CD4/CD8 ratio (r=0.44, p=0.01) correlated positively with forced expiratory volume in 1 s (FEV). Furthermore, CD3CD8 T-cells were negatively correlated with both FEV and FEV/forced vital capacity (r= -0.44, p=0.09 and r= -0.41, p=0.01, respectively).
Young adults with former BPD have a T-cell subset pattern in the airways resembling features of COPD. Our findings are compatible with the hypothesis that CD3CD8 T-cells are involved in mechanisms behind chronic airway obstruction in these patients.
支气管肺发育不良（BPD）是早产儿成年后慢性气道阻塞的危险因素。细胞毒性 T 细胞与 COPD 有关，但它们在 BPD 中的作用尚不清楚。
描述有 BPD 病史的成年人的气道 T 细胞亚群分布。
对 22 例有 BPD 病史的年轻成年人（中位年龄 19.6 岁）、22 例早产儿、22 例足月出生的过敏性哮喘患者和 24 例健康足月出生的对照者进行支气管肺泡灌洗（BAL）。使用流式细胞术分析 BAL 中的 T 细胞亚群。
研究组间细胞总数和 BAL 中的差异细胞计数相似。BPD 组 CD3CD8 T 细胞比例较高（p=0.005），CD3CD4 T 细胞比例降低（p=0.01），导致 CD4/CD8 比值降低（p=0.007），与健康对照组相比（中位数 2.2 5.3）。在 BPD 和早产儿研究对象中，CD3CD4 T 细胞（r=0.38，p=0.03）和 CD4/CD8 比值（r=0.44，p=0.01）均与 1 秒用力呼气量（FEV）呈正相关。此外，CD3CD8 T 细胞与 FEV 和 FEV/用力肺活量（r=-0.44，p=0.09 和 r=-0.41，p=0.01）均呈负相关。
有前 BPD 的年轻成年人的气道 T 细胞亚群模式类似于 COPD 的特征。我们的发现与 CD3CD8 T 细胞参与这些患者慢性气道阻塞机制的假设一致。
