Hannig E M, Williams T L, Leibowitz M J
Virology. 1986 Jul 15;152(1):149-58. doi: 10.1016/0042-6822(86)90380-6.
The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized (+) transcript (denoted m) of M1 dsRNA, neither of which contains 3'-terminal polyadenylate. We now show that there is variability in the length of the polyadenylate tracts of m transcripts synthesized in vitro by virions purified from either of the K1 diploid killer strains A364A X S7 or A364A X 1384. This variability reflects size differences seen in the corresponding M1 dsRNA genomes which, along with other data presented, localizes the variability in the length of M1 dsRNA to the adenine- and uracil-rich region.
来自酿酒酵母1型杀伤菌株的1.8千碱基对的M1双链（ds）RNA包含一个内部200碱基对的富含腺嘌呤和尿嘧啶的区域。我们之前已经证明，该区域主要由M1双链RNA正链上的腺嘌呤残基以及M1双链RNA的全长体外合成（+）转录本（记为m）组成，这两者均不包含3'-末端聚腺苷酸。我们现在表明，从K1二倍体杀伤菌株A364A×S7或A364A×1384中纯化的病毒体在体外合成的m转录本的聚腺苷酸尾长度存在变异性。这种变异性反映了在相应的M1双链RNA基因组中观察到的大小差异，结合其他所呈现的数据，将M1双链RNA长度的变异性定位到富含腺嘌呤和尿嘧啶的区域。
