Georgopoulos D E, Hannig E M, Leibowitz M J
Basic Life Sci. 1986;40:203-13. doi: 10.1007/978-1-4684-5251-8_16.
A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
利用体外转录本作为模板以及两条链的合成引物，通过逆转录合成了酵母杀伤病毒双链基因组M1-2区域的全长互补DNA（cDNA）拷贝。该序列缺乏任何长开放阅读框（ORF）。M1-2区域的内部部分包含与杀伤病毒双链RNA（dsRNA）的S3缺陷干扰突变体中M1-1同源区域的亚末端229个碱基相连的序列。因此，已确定S3中发生缺失的可能位点。
