Zhang Jiachan, Wang Changtao, An Quan, Quan Qianghua, Li Meng, Zhao Dan
Beijing Key Lab of Plant Resource Research and Development, College of chemistry and materials engineering, Beijing Technology and Business University, Beijing, 100048, People's Republic of China.
Yunnan Baiyao Group Co., Ltd., Kunming, 650000, People's Republic of China.
Clin Cosmet Investig Dermatol. 2022 Feb 15;15:217-235. doi: 10.2147/CCID.S348961. eCollection 2022.
Pathogenesis and persistence of many skin diseases are related to colonization. infection can cause varying degrees of changes in cell gene expression, resulting in complex changes in cell phenotype and finally changes in cell life activities.
The transcriptomes of healthy and -infected murine skin tissues were analyzed. We identified 638 differentially expressed genes (DEGs) in the infected tissues compared to the control samples, of which 324 were upregulated and 314 were downregulated, following the criteria of < 0.01 and |logFC| > 3. The DEGs were functionally annotated by Gene Ontology (GO), KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway and the protein-protein interaction (PPI) network analyses.
The upregulated DEGs were mainly enriched in GO terms, such as response to stimulus, immune system process and signal transduction, as well as in the complement and coagulation cascade pathway. Thus, infection likely activates these pathways to limit the influx of neutrophils and prevent skin damage. Four clusters were identified in the PPI network, and the major hubs were mainly related to cell cycle and proliferation, and mostly downregulated. The expression levels of Nox4, Mmrn1, Mcm5, Msx1 and Fgf5 mRNAs were validated by qRT-PCR and found to be consistent with the RNA-Seq data, confirming a strong correlation between the two approaches.
The identified genes and pathways are potential drug targets for treating skin inflammation caused by and should be investigated further.
许多皮肤疾病的发病机制和持续存在与定植有关。感染可导致细胞基因表达发生不同程度的变化，从而导致细胞表型的复杂变化，最终导致细胞生命活动的改变。
对健康和感染的小鼠皮肤组织的转录组进行分析。按照<0.01和|logFC|>3的标准，我们在感染组织中与对照样本相比鉴定出638个差异表达基因（DEG），其中324个上调，314个下调。通过基因本体论（GO）、京都基因与基因组百科全书（KEGG）途径和蛋白质-蛋白质相互作用（PPI）网络分析对DEG进行功能注释。
上调的DEG主要富集在GO术语中，如对刺激的反应、免疫系统过程和信号转导，以及补体和凝血级联途径。因此，感染可能激活这些途径以限制中性粒细胞的流入并防止皮肤损伤。在PPI网络中鉴定出四个簇，主要枢纽主要与细胞周期和增殖相关，且大多下调。通过qRT-PCR验证了Nox4、Mmrn1、Mcm5、Msx1和Fgf5 mRNA的表达水平，发现与RNA-Seq数据一致，证实了这两种方法之间有很强的相关性。
鉴定出的基因和途径是治疗由[感染源未明确]引起的皮肤炎症的潜在药物靶点，应进一步研究。
