Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, People's Republic of China.
College of Food Science, South China Agricultural University, Guangzhou, People's Republic of China.
Pol J Microbiol. 2021 Dec;70(4):447-459. doi: 10.33073/pjm-2021-041. Epub 2021 Dec 23.
is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of fruits inhibit the planktonic growth and initial biofilm formation of in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of fruits can control infections and sought to understand the mode of action of these extracts on .
是许多不同临床感染的病原体。果实的粗水提物以剂量依赖的方式抑制浮游生长和初始生物膜形成。此外，随着水提物浓度的增加，生物膜拓扑结构变得稀疏和减少。RNA-Seq 分析显示，暴露于 0.25 g/ml 提取物后，有 532 个差异表达基因（DEGs）；其中 319 个上调，213 个下调。大多数 DEGs 在基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径中被归类为丰富的亚组。最后，对果实的水提物进行非靶向 UHPLC-MS/MS 分析，在正离子和负离子电喷雾电离模式下显示出高度复杂的图谱。提取物主要由脂质和类脂分子、有机酸及其衍生物、苯丙素、聚酮、有机杂环化合物和芳香族化合物组成，通过丰富的脂质图谱和 KEGG 途径进行注释。总的来说，这项研究提供了证据表明果实的水提物可以控制感染，并试图了解这些提取物对的作用模式。
