Tortosa María, Velasco Pablo, Rodríguez Víctor Manuel, Cartea María Elena
Group of Genetics, Breeding and Biochemistry of Brassicas, Misión Biológica de Galicia, Spanish Council for Scientific Research (CSIC), Pontevedra, Spain.
Front Plant Sci. 2022 Feb 8;12:781984. doi: 10.3389/fpls.2021.781984. eCollection 2021.
Understanding plant's response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, "omic" approaches are heralded as valuable technologies. In this work, combining isobaric tags for relative and absolute quantification (iTRAQ) technology with mass spectrometry, the proteomes from leaves of plants infected with pv. (Xcc), and control plants at two different post-infection times were compared. Stronger proteomic changes were obtained at 12 days post-infection in comparison with 3 days. The responses observed involved different cell processes, from primary metabolism, such as photosynthesis or photorespiration, to other complex processes such as redox homeostasis, hormone signaling, or defense mechanisms. Most of the proteins decreased in the earlier response were involved in energetic metabolism, whereas later response was characterized by a recovery of primary metabolism. Furthermore, our results indicated that proteolysis machinery and reactive oxygen species (ROS) homeostasis could be key processes during this plant-pathogen interaction. Current data provide new insights into molecular mechanisms that may be involved in defense responses of to Xcc.
了解植物对病原菌的反应机制是培育抗病作物品种和实现更高农业产量的基础。在这方面，“组学”方法被誉为有价值的技术。在这项工作中，将用于相对和绝对定量的等压标签（iTRAQ）技术与质谱相结合，比较了感染野油菜黄单胞菌（Xcc）的植物叶片和对照植物在两个不同感染后时间点的蛋白质组。与感染后3天相比，感染后12天获得了更强的蛋白质组变化。观察到的反应涉及不同的细胞过程，从光合作用或光呼吸等初级代谢到氧化还原稳态、激素信号传导或防御机制等其他复杂过程。早期反应中大多数蛋白质减少，这些蛋白质参与能量代谢，而后期反应的特征是初级代谢的恢复。此外，我们的结果表明，蛋白水解机制和活性氧（ROS）稳态可能是这种植物-病原体相互作用过程中的关键过程。目前的数据为可能参与甘蓝对Xcc防御反应的分子机制提供了新的见解。
