Villeth Gabriela R C, Carmo Lílian S T, Silva Luciano Paulino, Santos Mateus Figueiredo, de Oliveira Neto Osmundo Brilhante, Grossi-de-Sá Maria Fátima, Ribeiro Igor Sousa, Dessaune Suelen Nogueira, Fragoso Rodrigo Rocha, Franco Octávio L, Mehta Angela
Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
Embrapa Hortaliças, Gama, DF, Brazil.
J Proteomics. 2016 Jun 30;143:278-285. doi: 10.1016/j.jprot.2016.01.014. Epub 2016 Jan 26.
Cruciferous plants are important edible vegetables widely consumed around the world, including cabbage, cauli-flower and broccoli. The main disease that affects crucifer plants is black rot, caused by Xanthomonas campestris pv. campestris (Xcc). In order to better understand this specific plant-pathogen interaction, proteins responsive to Xcc infection in resistant (União) and susceptible (Kenzan) Brassica oleracea cultivars were investigated by 2-DE followed by mass spectrometry. A total of 47 variable spots were identified and revealed that in the susceptible interaction there is a clear reduction in the abundance of proteins involved in energetic metabolism and defense. It was interesting to observe that in the resistant interaction, these proteins showed an opposite behavior. Based on our results, we conclude that resistance is correlated with the ability of the plant to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for an active defense. As a follow-up study, qRT-PCR analysis of selected genes was performed and revealed that most genes showed an up-regulation trend from 5 to 15days after inoculation (DAI), showing highest transcript levels at 15DAI. These results revealed the gradual accumulation of transcripts providing a more detailed view of the changes occurring during different stages of the plant-pathogen interaction.
In this study we have compared cultivars of Brassica oleracea (cabbage), susceptible and resistant to black rot, by using the classical 2-DE approach. We have found that resistance is correlated with the ability of the plant to keep sufficient photosynthesis metabolism activity to provide energy supplies necessary for an active defense.
十字花科植物是世界各地广泛食用的重要蔬菜，包括卷心菜、花椰菜和西兰花。影响十字花科植物的主要病害是黑腐病，由野油菜黄单胞菌野油菜致病变种（Xcc）引起。为了更好地理解这种特定的植物-病原体相互作用，通过二维电泳（2-DE）和质谱分析研究了甘蓝抗性品种（尤尼奥）和感病品种（肯赞）中对Xcc感染有反应的蛋白质。共鉴定出47个可变斑点，结果表明，在感病相互作用中，参与能量代谢和防御的蛋白质丰度明显降低。有趣的是，在抗性相互作用中，这些蛋白质表现出相反的行为。根据我们的结果，我们得出结论，抗性与植物保持足够的光合作用代谢活性以提供主动防御所需能量供应的能力相关。作为后续研究，对选定基因进行了定量逆转录聚合酶链反应（qRT-PCR）分析，结果表明，大多数基因在接种后5至15天呈现上调趋势，在接种后15天转录水平最高。这些结果揭示了转录本的逐渐积累，更详细地展示了植物-病原体相互作用不同阶段发生的变化。
在本研究中，我们使用经典的二维电泳方法比较了甘蓝（卷心菜）对黑腐病的感病和抗病品种。我们发现，抗性与植物保持足够的光合作用代谢活性以提供主动防御所需能量供应的能力相关。
