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两种染色技术在犬精子形态分析的手动和自动分析中的比较。

Comparison of two staining techniques on the manual and automated canine sperm morphology analysis.

机构信息

Department of Large Animal Diseases with Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences WULS - SGGW, Warsaw, Poland.

Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Warsaw, Poland.

出版信息

Reprod Domest Anim. 2022 Jun;57(6):678-684. doi: 10.1111/rda.14100. Epub 2022 Mar 4.

DOI:10.1111/rda.14100
PMID:35212033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9311200/
Abstract

Detailed and direct analysis of semen, including sperm morphology, enables a diagnosis of male fertility. This study aimed to describe an economical and verified protocol for canine spermiograms and compare the effectiveness of Sperm Stain and Sperm Blue (Microptic, Spain) in veterinary practice. Sperm assessment was conducted manually, using a standard optical microscope, and via computerized semen analysis using the SCA CASA (Sperm Class Analyzer CASA System-MICROPTIC, Spain). This study showed that Sperm Blue is a better solution for computerized sperm quality analysis of healthy dogs. At the same time, Sperm Stain turned out to be more helpful in identifying specific morphological defects of sperm. Automated canine sperm morphology analysis worked better with Sperm Blue stain, but Sperm Stain simplified manual evaluation of various organelles' defects. Standard, manual examination is more error-prone for an inexperienced andrology technician, but it seems to be still a gold standard technique for canine sperm assessment.

摘要

详细和直接的精液分析，包括精子形态学分析，可以诊断男性生育力。本研究旨在描述一种经济且经过验证的犬精液分析方案，并比较 Sperm Stain 和 Sperm Blue（西班牙 Microptic）在兽医实践中的效果。使用标准光学显微镜和 SCA CASA（精子类分析器 CASA 系统-MICROPTIC，西班牙）进行手动和计算机化的精液分析。本研究表明，Sperm Blue 是一种更好的用于健康犬计算机化精子质量分析的解决方案。同时，Sperm Stain 更有助于识别精子的特定形态缺陷。自动化犬精子形态分析与 Sperm Blue 染色配合得更好，但 Sperm Stain 简化了对各种细胞器缺陷的手动评估。对于经验不足的男科技术员来说，标准的手动检查更容易出错，但它似乎仍然是犬精子评估的金标准技术。

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