Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, Washington, USA.
Department of Biomedical Engineering, State University of New York at Buffalo, 208 Bonner Hall, Buffalo, New York, USA.
Lab Chip. 2022 Mar 29;22(7):1354-1364. doi: 10.1039/d1lc01142a.
Minimally invasive core needle biopsies for medical diagnoses have become increasingly common for many diseases. Although tissue cores can yield more diagnostic information than fine needle biopsies and cytologic evaluations, there is no rapid assessment at the point-of-care for intact tissue cores that is low-cost and non-destructive to the biopsy. We have developed a proof-of-concept 3D printed millifluidic histopathology lab-on-a-chip device to automatically handle, process, and image fresh core needle biopsies. This device, named CoreView, includes modules for biopsy removal from the acquisition tool, transport, staining and rinsing, imaging, segmentation, and multiplexed storage. Reliable removal from side-cutting needles and bidirectional fluid transport of core needle biopsies of five tissue types has been demonstrated with 0.5 mm positioning accuracy. Automation is aided by a MATLAB-based biopsy tracking algorithm that can detect the location of tissue and air bubbles in the channels of the millifluidic chip. With current and emerging optical imaging technologies, CoreView can be used for a rapid adequacy test at the point-of-care for tissue identification as well as glomeruli counting in renal core needle biopsies.
微创核心针活检已成为许多疾病的常见诊断方法。虽然组织芯比细针活检和细胞学评估能提供更多的诊断信息，但目前还没有一种低成本、非破坏性的即时评估方法，用于对完整的组织芯进行快速评估。我们已经开发出一种概念验证的 3D 打印微流控组织病理学芯片实验室设备，用于自动处理、处理和成像新鲜的核心针活检。该设备名为 CoreView，包括从采集工具中取出活检、运输、染色和冲洗、成像、分割以及多路复用存储等模块。该设备已经证明可以可靠地从侧切针中取出活检，并对 5 种组织类型的核心针活检进行双向流体传输，定位精度为 0.5 毫米。MATLAB 为基础的活检跟踪算法有助于实现自动化，该算法可以检测微流控芯片通道中的组织和气泡的位置。随着当前和新兴的光学成像技术的发展，CoreView 可以用于即时评估组织识别的充分性，以及肾脏核心针活检中的肾小球计数。
