Department of Pathology and Laboratory Medicine, University of California Davis Medical Center, Sacramento, CA, USA.
Department of Biochemistry and Molecular Medicine, University of California Davis Medical Center, Sacramento, CA, USA.
Nat Biomed Eng. 2017 Dec;1(12):957-966. doi: 10.1038/s41551-017-0165-y. Epub 2017 Dec 4.
Histological examination of tissues is central to the diagnosis and management of neoplasms and many other diseases and is a foundational technique for preclinical and basic research. However, commonly used bright-field microscopy requires prior preparation of micrometre-thick tissue sections mounted on glass slides-a process that can require hours or days, contributes to cost and delays access to critical information. Here, we introduce a simple, non-destructive slide-free technique that, within minutes, provides high-resolution diagnostic histological images resembling those obtained from conventional haematoxylin and eosin histology. The approach, which we named microscopy with ultraviolet surface excitation (MUSE), can also generate shape and colour-contrast information. MUSE relies on ~280 nm ultraviolet light to restrict the excitation of conventional fluorescent stains to tissue surfaces and it has no significant effects on downstream molecular assays (including fluorescence in situ hybridization and RNA sequencing). MUSE promises to improve the speed and efficiency of patient care in both state-of-the-art and low-resource settings and to provide opportunities for rapid histology in research.
组织的组织学检查是肿瘤和许多其他疾病的诊断和治疗的核心，也是临床前和基础研究的基础技术。然而，常用的明场显微镜需要预先制备微米厚的组织切片，这些切片安装在载玻片上，这个过程可能需要数小时或数天，不仅增加了成本，还延迟了获取关键信息的时间。在这里，我们介绍了一种简单、非破坏性的免载玻片技术，它可以在几分钟内提供类似于传统苏木精和伊红染色的高分辨率诊断组织学图像。我们将这种方法命名为紫外表面激发显微镜（MUSE），它还可以生成形状和颜色对比信息。MUSE 依赖于~280nm 的紫外光将传统荧光染料的激发限制在组织表面，对下游分子检测（包括荧光原位杂交和 RNA 测序）没有显著影响。MUSE 有望提高在先进和资源匮乏环境中患者护理的速度和效率，并为研究中的快速组织学提供机会。
