Department of Applied Life Science, Graduate School, Konkuk University, Chungju, South Korea.
Department of Pathophysiology, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, People's Republic of China.
Methods Mol Biol. 2022;2459:137-147. doi: 10.1007/978-1-0716-2144-8_14.
Sepsis is a complex disorder related to dysregulation of the host response to infection and is a major health problem worldwide owing to its high mortality rates. However, the exact mechanisms causing sepsis remains unclear because of the complexity of the underlying pathways. Dysregulation of non-canonical NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome activation induces septic shock by promoting pyroptosis and pro-inflammatory cytokine production (e.g., interleukin-1β) via caspase-11 and Gasdermin-D. Herein, we describe a rapid, simple method for evaluation of the degree of sepsis by investigating non-canonical inflammasome activation in both in vitro and in vivo models. The method is expected to be useful for testing and screening drugs for the treatment of sepsis.
脓毒症是一种与宿主对感染的反应失调有关的复杂疾病，由于其高死亡率，是一个全球性的主要健康问题。然而，由于潜在途径的复杂性，导致脓毒症的确切机制仍不清楚。非经典 NOD、LRR 和 pyrin 结构域包含蛋白 3（NLRP3）炎性小体激活的失调通过胱天蛋白酶-11 和 Gasdermin-D 促进细胞焦亡和促炎细胞因子（如白细胞介素-1β）的产生来诱导感染性休克。在此，我们描述了一种通过研究体外和体内模型中非经典炎性小体激活来评估脓毒症严重程度的快速、简单的方法。该方法有望用于测试和筛选治疗脓毒症的药物。
