Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China.
Sensors (Basel). 2022 Feb 10;22(4):1346. doi: 10.3390/s22041346.
A single-molecule detection method was developed for nucleic acids based on mass spectrometry counting single liposome particles. Before the appearance of symptoms, a negligible amount of nucleic acids and biomarkers for the clinical diagnosis of the disease were already present. However, it is difficult to detect extremely low concentrations of nucleic acids using the current methods. Hence, the establishment of an ultra-sensitive nucleic acid detection technique is urgently needed. Herein, magnetic beads were used to capture target nucleic acids, and liposome particles were employed as mass tags for single-particle measurements. Liposomes were released from magnetic beads via photocatalytic cleavage. Hence, one DNA molecule corresponded to one liposome particle, which could be counted using mass spectrometric measurement. The ultrasensitive detection of DNA (10 M) was achieved using this method.
基于质谱计数单个脂质体颗粒，开发了一种用于核酸的单分子检测方法。在出现症状之前，已经存在极少量的核酸和疾病临床诊断的生物标志物。然而，目前的方法很难检测到极低浓度的核酸。因此，迫切需要建立一种超灵敏的核酸检测技术。在此，使用磁珠捕获靶核酸，并将脂质体用作单颗粒测量的质量标记物。通过光催化裂解从磁珠上释放脂质体。因此，一个 DNA 分子对应一个脂质体颗粒，可以通过质谱测量进行计数。该方法实现了对 DNA（10 M）的超灵敏检测。
