Ghosh Arijit, Saha Indraneel, Fujita Masayuki, Debnath Subhas Chandra, Hazra Alok Kumar, Adak Malay Kumar, Hasanuzzaman Mirza
Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani, Nadia 741235, West Bengal, India.
Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Plants (Basel). 2022 Feb 14;11(4):513. doi: 10.3390/plants11040513.
Capsicum is one of the most perishable fruit which undergo rapid loss of commercial value during postharvest storage. In this experiment our aim is to evaluate the effect of photoactivated TiO nano-particle complexed with chitosan or TiO-nanocomposite (TiO-NC) on extension self-life of fruit and its effect on related morphological, physiological and molecular attributes at room temperature (25 °C). Initially, TiO-NC coated fruits recorded superior maintenance of total soluble solids accumulation along with retention of firmness, cellular integrity, hydration, color etc. On the extended period of storage, fruit recorded a lower bioaccumulation of TiO in comparison to metallic silver over the control. On the level of gene expression for ethylene biosynthetic and signaling the TiO-NC had more regulation, however, discretely to moderate the ripening. Thus, ACC synthase and oxidase recorded a significantly better downregulation as studied from fruit pulp under TiO-NC than silver. On the signaling path, the transcripts for Ca and Ca were less abundant in fruit under both the treatment when studied against control for 7 d. The reactive oxygen species (ROS) was also correlated to retard the oxidative lysis of polyamine oxidation by diamine and polyamine oxidase activity. The gene expression for hydrolytic activity as non-specific esterase had corroborated the development of essential oil constituents with few of those recorded in significant abundance. Therefore, TiO-NC would be reliable to induce those metabolites modulating ripening behavior in favor of delayed ripening. From gas chromatography-mass spectrometry (GC-MS) analysis profile of all tested essential oil constituents suggesting positive impact of TiO-NC on shelf-life extension of fruit. Our results indicated the potentiality of TiO-NC in postharvest storage those may connect ethylene signaling and ROS metabolism in suppression of specific ripening attributes.
辣椒是最易腐坏的水果之一，在采后储存期间其商业价值会迅速丧失。在本实验中，我们的目的是评估与壳聚糖复合的光活化二氧化钛纳米颗粒或二氧化钛纳米复合材料（TiO-NC）对果实延长保质期的影响，以及其在室温（25°C）下对相关形态、生理和分子特性的影响。最初，涂有TiO-NC的果实记录显示，其总可溶性固形物积累得到了更好的维持，同时果实硬度、细胞完整性、水分含量、颜色等也得以保持。在延长储存期后，与对照相比，果实中TiO的生物积累量低于金属银。在乙烯生物合成和信号传导的基因表达水平上，TiO-NC具有更多调控作用，但只是适度调节成熟过程。因此，从TiO-NC处理的果肉中研究发现，ACC合成酶和氧化酶的下调效果明显优于银处理。在信号传导途径方面，与对照相比，处理7天后，两种处理果实中钙和钙的转录本含量均较少。活性氧（ROS）也与抑制二胺和多胺氧化酶活性引起的多胺氧化的氧化裂解相关。作为非特异性酯酶的水解活性的基因表达证实了精油成分的形成，其中一些成分含量显著。因此，TiO-NC有望诱导那些调节成熟行为的代谢物，从而有利于延迟成熟。从气相色谱-质谱（GC-MS）分析所有测试精油成分的图谱表明，TiO-NC对果实货架期延长有积极影响。我们的结果表明，TiO-NC在采后储存中具有潜力，可能通过连接乙烯信号传导和ROS代谢来抑制特定的成熟特性。
