Laboratory of Genome Editing, Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia.
Viruses. 2022 Feb 21;14(2):437. doi: 10.3390/v14020437.
Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
甲型流感病毒（IAV）会引起呼吸道感染，影响到不同年龄组的数百万人，并且可能导致急性呼吸窘迫综合征。目前，宿主基因、受体和其他对 IAV 复制至关重要的细胞成分正在被积极研究。CRISPR/Cas9 系统是一种最方便和易于使用的基因组编辑工具，可以促进这些研究。该工具可以调节病毒和宿主细胞基因的表达，以增强或削弱病毒的进入和复制。本综述考虑了基因组编辑系统对细胞（人源和鸡源）中特定靶基因的影响，以及这些变化对流感病毒生命周期和病毒粒子产生效率的影响。
