The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Int J Mol Sci. 2022 Feb 14;23(4):2099. doi: 10.3390/ijms23042099.
The () gene family affects plant architecture, panicle structure, and grain development, representing key genes for crop improvements. The objective of the present study is to utilize the well characterized s' functions in rice to facilitate the functional genomics of genes. To achieve these goals, we combined several approaches, including genome-wide analysis of s, comparative genomic analysis, expression profiling, and functional study of in rice. We established the orthologous relationships of 56 genes with the corresponding s, laying a foundation for the comparison of known functions between wheat and rice. Some s exhibited different spatial-temporal expression patterns when compared to their rice orthologs, thus implicating functional divergence. /// were identified to respond to different abiotic stresses through the combination of RNA-seq and qPCR expression analysis. Additionally, ectopic expression of in rice promotes heading dates, affects leaf and stem development, and leads to smaller panicles and decreased yields per panicle. In conclusion, our work provides useful information toward cataloging of the functions of s, emphasized the conservation and divergence between s and s, and identified the important genes for wheat improvement.
拟南芥 SBP 基因家族影响植物的结构、穗结构和谷物发育，是作物改良的关键基因。本研究的目的是利用拟南芥中 SBP 基因的功能特征，促进 SBP 基因的功能基因组学研究。为了实现这些目标，我们结合了多种方法，包括 SBP 基因的全基因组分析、比较基因组分析、表达谱分析以及在水稻中的功能研究。我们建立了 56 个 SBP 基因与相应的拟南芥 SBP 基因的直系同源关系，为比较小麦和水稻中已知 SBP 基因功能奠定了基础。一些 SBP 基因与它们的水稻直系同源基因相比表现出不同的时空表达模式，暗示了功能的分化。通过 RNA-seq 和 qPCR 表达分析的结合，鉴定到了一些 SBP 基因可以响应不同的非生物胁迫。此外，在水稻中异位表达 ，可以促进抽穗期，影响叶片和茎的发育，导致穗变小，每穗产量降低。总之，我们的工作为 SBP 基因的功能分类提供了有用的信息，强调了 SBP 基因和 SBP 基因之间的保守性和分化，以及鉴定了对小麦改良重要的 SBP 基因。
