Centre de Recherche Saint-Antoine (CRSA), INSERM, Sorbonne Université, F-75012 Paris, France.
Laboratoire de Réactivité de Surface, CNRS, Sorbonne Université, F-75005 Paris, France.
Int J Mol Sci. 2022 Feb 18;23(4):2297. doi: 10.3390/ijms23042297.
Classical treatments of shoulder instability are associated with recurrence. To determine whether the modification of the capsule properties may be an alternative procedure, the effect of crosslinking treatment on the structure and mechanical properties of diseased human shoulder capsules was investigated. Joint capsules harvested from patients during shoulder surgery (n = 5) were treated or not with UV and/or riboflavin (0.1%, 1.0% and 2.5%). The structure and the mechanical properties of the capsules were determined by atomic force microscopy. The effect of treatments on cell death was investigated. Collagen fibrils were well-aligned and adjacent to each other with a D-periodicity of 66.9 ± 3.2 nm and a diameter of 71.8 ± 15.4 nm in control untreated capsules. No effect of treatments was observed on the organization of the collagen fibrils nor on their intrinsic characteristics, including D-periodicity or their mean diameter. The treatments also did not induce cell death. In contrast, UV + 2.5% riboflavin induced capsule stiffness, as revealed by the increased Young's modulus values ( < 0.0001 for each patient). Our results showed that the crosslinking procedure changed the biomechanics of diseased capsules, while keeping their structural organisation unchanged at the single fibril level. The UV/riboflavin crosslinking procedure may be a promising way to preserve the functions of collagen-based tissues and tune their elasticity for clinically relevant treatments.
经典的肩关节不稳定治疗方法与复发有关。为了确定是否可以通过改变囊组织的特性来替代手术，研究了交联处理对病变的人肩关节囊的结构和力学性能的影响。在肩关节手术中，从患者身上采集的关节囊（n=5）经过或未经紫外线（UV）和/或核黄素（0.1%、1.0%和 2.5%）处理。采用原子力显微镜（AFM）测定囊的结构和力学性能。研究了处理对细胞死亡的影响。在对照未处理的囊组织中，胶原纤维排列整齐，彼此相邻，D-周期性为 66.9±3.2nm，直径为 71.8±15.4nm。处理对胶原纤维的排列或其固有特性（包括 D-周期性或平均直径）没有影响。处理也没有诱导细胞死亡。相反，UV+2.5%核黄素处理会导致囊的刚性增加，这反映在杨氏模量值的增加（每个患者<0.0001）。我们的研究结果表明，交联过程改变了病变囊的生物力学特性，同时在单纤维水平上保持其结构组织不变。UV/核黄素交联处理可能是一种有前途的方法，可以保留基于胶原蛋白组织的功能，并调整其弹性，以满足临床相关的治疗需求。
