Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Tijuana - Ensenada No. 3918, Zona Playitas, Ensenada, Baja California CP 22860, México.
Instituto de Investigaciones Oceanológicas (IIO), Universidad Autónoma de Baja California (UABC), Carretera Ensenada-Tijuana No. 3917, Fraccionamiento Playitas, Ensenada, Baja California CP 22860, Mexico.
Gen Comp Endocrinol. 2022 May 1;320:114000. doi: 10.1016/j.ygcen.2022.114000. Epub 2022 Feb 23.
The optic glands (OG) of cephalopods are a source of molecules associated with the control of reproductive traits and lifecycle events such as sexual maturation, reproductive behavior, feeding, parental care, and senescence. However, little is known about the role of the optic gland in Octopus maya adults during mating and egg laying. RNA sequencing, de novo transcriptome assembly, ubiquity and differential expression analysis were performed. First, we analyzed the expression patterns of transcripts commonly associated with OG regulatory functions to describe their possible role once the maturation of the gonad is complete. The transcriptomic profiles of the optic gland of both sexes were compared with emphasis on the signaling pathways involved in the dimorphism of reproductive traits. Results suggest that in the OG of males, the reproductive condition (mated or non-mated) did not affect the general expression profile. In contrast, more differentially expressed genes were observed in females. In mated females, the mRNA metabolic process and the response to norepinephrine were enriched, suggesting a high cellular activity in preparation for the laying of the embryos. Whereas in egg-laying females, energetic and metabolic processes were the most represented, including the oxidation-reduction process. Finally, the gene expression patterns in senescence females suggest a physiological response to starvation as well as upregulation of genes involved retrotransposon activity. In conclusion, more substantial fluctuations in gene expression were observed in the optic glands of the fertilized females compared to the males. Such differences might be associated with the regulation of the egg-laying and the onset of senescence.
头足类动物的眼柄是与生殖特征和生命周期事件（如性成熟、生殖行为、摄食、亲代养育和衰老）控制相关的分子的来源。然而，对于在交配和产卵期间，章鱼眼柄在成年章鱼中的作用知之甚少。进行了 RNA 测序、从头转录组组装、泛素化和差异表达分析。首先，我们分析了与 OG 调节功能相关的常见转录本的表达模式，以描述在性腺成熟完成后它们可能的作用。比较了雌雄眼柄的转录组谱，重点是涉及生殖特征二态性的信号通路。结果表明，在雄性眼柄中，生殖状况（交配或未交配）不会影响一般表达谱。相比之下，在雌性中观察到更多差异表达的基因。在交配的雌性中，mRNA 代谢过程和对去甲肾上腺素的反应被富集，表明在为胚胎产卵做准备时细胞活动很高。而在产卵的雌性中，能量和代谢过程是最具代表性的，包括氧化还原过程。最后，衰老雌性的基因表达模式表明对饥饿的生理反应以及涉及逆转座子活性的基因的上调。总之，与雄性相比，受精雌性眼柄中的基因表达波动更大。这些差异可能与产卵和衰老的开始的调节有关。
