Traditional vaccination relies on subcutaneous injection or intramuscular injection, which requires professional medical personnel and is accompanied by the risk of needle-related diseases and injuries. Therefore, to promote immunization coverage and reduce costs, it is necessary to provide a new method of vaccine administration. Dissolving microneedle (DMN) has been proposed as an alternative to hypodermic needles, providing prospects for self-inoculation and increasing immunogenicity by directly targeting skin dendritic cells. This study reported the successful preparation and characterization of Bletilla striata polysaccharide microneedles (BMNs) and investigated the potential of this natural material-based DMN as a vaccine carrier. The prepared BMNs exhibited more excellent mechanical properties and stability compared with microneedles made of hyaluronic acid and polyvinyl alcohol. BMNs had good cell compatibility, low bacterial skin permeability, slight irritation to the skin, and no infection or inflammation in the body. In addition, as shown by circular dichroism, the molecular structure of the antigen ovalbumin (OVA) loaded in BMN did not change during storage for 21 days. The Franz diffusion cell experiment showed 76.74% of OVA was released to the skin within 3 h. These encouraging findings indicate that the BMNs can be a promising tool for effective vaccine delivery.