Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Bioresour Technol. 2022 Apr;350:126899. doi: 10.1016/j.biortech.2022.126899. Epub 2022 Feb 22.
Fatty acid ethyl esters (FAEEs) have gained increasing attention as a replacement for traditional fossil fuels in the recent years. Here, we report the efficient upgrading of ethanol to FAEEs from Pseudomonas putida KT2440, using ethanol as the sole carbon source. First, the wax synthase (WS) encoded by the atfA gene from Acinetobacter baylyi ADP1 was expressed in P. putida KT2440. Second, the flux from ethanol towards acetyl-CoA was increased by expression of the acetaldehyde dehydrogenase (ada) from Dickeya zeae. By using dodecane overlay to capture FAEEs, 1.2 g/L of FAEEs with a yield of 152.09 mg FAEEs/g ethanol were produced. Culture optimization enhanced the FAEEs contents up to 1.6 g/L in shake flask and 4.3 g/L in a fed-batch fermenter. In summary, our study provides a basis for combining the bioethanol production process with the efficient upgrading of ethanol to biodiesel.
近年来，脂肪酸乙酯 (FAEE) 作为传统化石燃料的替代品受到了越来越多的关注。在这里，我们报告了使用乙醇作为唯一碳源，从恶臭假单胞菌 KT2440 中高效地将乙醇升级为 FAEE。首先，在恶臭假单胞菌 KT2440 中表达了来自鲍曼不动杆菌 ADP1 的 atfA 基因编码的蜡合酶 (WS)。其次，通过表达来自玉米细菌性枯萎病菌的乙醛脱氢酶 (ada)，增加了乙醇向乙酰辅酶 A 的通量。通过使用十二烷覆盖物捕获 FAEE，以 152.09mg FAEE/g 乙醇的产率生产了 1.2g/L 的 FAEE。通过摇瓶和补料分批发酵罐中的培养优化，FAEE 含量分别提高到 1.6g/L 和 4.3g/L。总之，我们的研究为将生物乙醇生产过程与乙醇高效升级为生物柴油相结合提供了基础。
