Sun Manman, Gao Alex Xiong, Liu Xiuxia, Bai Zhonghu, Wang Peng, Ledesma-Amaro Rodrigo
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Institute of Hefei Artificial Intelligence Breeding Accelerator, Hefei, 230000, China.
Biotechnol Biofuels Bioprod. 2024 Aug 19;17(1):115. doi: 10.1186/s13068-024-02546-w.
Industrial biotechnology heavily relies on the microbial conversion of carbohydrate substrates derived from sugar- or starch-rich crops. This dependency poses significant challenges in the face of a rising population and food scarcity. Consequently, exploring renewable, non-competing carbon sources for sustainable bioprocessing becomes increasingly important. Ethanol, a key C2 feedstock, presents a promising alternative, especially for producing acetyl-CoA derivatives. In this review, we offer an in-depth analysis of ethanol's potential as an alternative carbon source, summarizing its distinctive characteristics when utilized by microbes, microbial ethanol metabolism pathway, and microbial responses and tolerance mechanisms to ethanol stress. We provide an update on recent progress in ethanol-based biomanufacturing and ethanol biosynthesis, discuss current challenges, and outline potential research directions to guide future advancements in this field. The insights presented here could serve as valuable theoretical support for researchers and industry professionals seeking to harness ethanol's potential for the production of high-value products.
工业生物技术严重依赖于源自富含糖或淀粉作物的碳水化合物底物的微生物转化。面对人口增长和粮食短缺，这种依赖性带来了重大挑战。因此，探索可再生、非竞争性的碳源以实现可持续生物加工变得越来越重要。乙醇作为一种关键的C2原料，是一种很有前景的替代品，特别是用于生产乙酰辅酶A衍生物。在这篇综述中，我们深入分析了乙醇作为替代碳源的潜力，总结了其被微生物利用时的独特特性、微生物乙醇代谢途径以及微生物对乙醇胁迫的响应和耐受机制。我们介绍了基于乙醇的生物制造和乙醇生物合成的最新进展，讨论了当前的挑战，并概述了潜在的研究方向，以指导该领域未来的发展。本文所提供的见解可为寻求利用乙醇生产高价值产品潜力的研究人员和行业专业人士提供有价值的理论支持。
