Department of Physiology, Uskudar University Faculty of Medicine, Istanbul, Turkey.
Department of Medical Biochemistry, Faculty of Medicine Istinye University, Istanbul, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 May 15;273:121029. doi: 10.1016/j.saa.2022.121029. Epub 2022 Feb 18.
Polycystic ovarian syndrome (PCOS) is a disease, which causes infertility in women. The factors for the development of the disease are still not well understood and diagnostic methods need to be improved. Therefore, in this study, Raman spectroscopy as a potential diagnostic tool, was investigated and spectra of blood serum were collected from PCOS and healthy women. The obtained spectra showed distinct changes in intensities as well as shift of peaks for the blood serum collected from PCOS compared to healthy individuals. Partial Last Square (PLS) analysis and Principal Component Analysis (PCA) allowed to determine that Raman shifts of amides (1500 - 1700 cm) and CH, CH lipid groups (2700 - 3000 cm), could be thus used as potential PCOS markers. Furthermore, the Pearson correlation test showed a strong correlation between hormones (lutropin (LH), prolactin (PRL), follicle-stimulating (FSH), dehydroepiandrosterone (DHEAS), thyroid-stimulating (TSH), Estradiol) and lipids, as well as between hormones and protein functional groups in PCOS women, compared to the control. These results show, that the lipid and protein balance could be potentially applied as a helpful PCOS marker in Raman spectra.
多囊卵巢综合征（PCOS）是一种导致女性不孕的疾病。该疾病的发展因素尚不清楚，诊断方法需要改进。因此，在这项研究中，我们研究了拉曼光谱作为一种潜在的诊断工具，并从 PCOS 患者和健康女性中采集了血清的拉曼光谱。与健康个体相比，从 PCOS 患者中采集的血清的拉曼光谱在强度和峰位移方面表现出明显的变化。偏最小二乘（PLS）分析和主成分分析（PCA）表明，酰胺（1500-1700 cm）和 CH、CH 脂质基团（2700-3000 cm）的拉曼位移可作为潜在的 PCOS 标志物。此外，Pearson 相关检验显示，与对照组相比，PCOS 患者的激素（促黄体激素（LH）、催乳素（PRL）、促卵泡激素（FSH）、脱氢表雄酮（DHEAS）、促甲状腺激素（TSH）、雌二醇）和脂质之间，以及激素和蛋白质功能基团之间存在很强的相关性。这些结果表明，脂质和蛋白质平衡可能可以作为拉曼光谱中有用的 PCOS 标志物。
