The National Engineering Research Center of Crop Molecular Breeding, MARA Key Laboratory of Soybean Biology (Beijing), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Food Chem. 2022 Aug 1;384:132520. doi: 10.1016/j.foodchem.2022.132520. Epub 2022 Feb 21.
Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-Hfolate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH-Hfolate correlated positively with seed protein (r = 0.24***) and negatively with oil (r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-Hfolate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.
大豆是叶酸的丰富来源。我们通过 HPLC-MS/MS 优化了从大豆种子中提取和检测叶酸的方法，并分析了 1074 个品系的叶酸含量和组成。总叶酸含量范围为 64.51 至 691.24μg/100g 鲜重，变化幅度为 10 倍，鉴定出 60 个总叶酸含量超过 400μg/100g 的优良品系。最丰富的成分是 5-CHO-Hfolate，平均占总叶酸含量的 60%。种皮颜色、种子重量、生态区和品系类型显著影响大豆叶酸含量。此外，5-CH-Hfolate 与种子蛋白质呈正相关（r=0.24***)，与油呈负相关（r=-0.26***）。根据起源品系的叶酸地理分布表明，来自中国东北地区的品系含有更高的总叶酸和 5-CHO-Hfolate。本研究全面深入地了解了大豆的叶酸特征，这将有利于为提高叶酸含量而进行的大豆育种。
