Department of Pharmacology, Faculty of Basic Medical Sciences, Obafemi Awolowo College of Health Sciences, OlabisiOnabanjo University, Sagamu Campus, Ogun State, Nigeria.
Faculty of Health, Canadian Imperial College, Alberta, Canada.
Drug Metab Pers Ther. 2022 Feb 24;37(3):305-314. doi: 10.1515/dmpt-2021-0166. eCollection 2022 Sep 1.
Ethno-botanical surveys uncovered the use of in the management of mental health abnormalities. Antidepressant- and anxiolytic-like activities of hydroethanol leaf extract of (HLDS) in mice and its possible mechanism of action were investigated in this study.
The hole-board test (HBT), open field test (OFT), elevated plus maze test (EPMT), and social interaction test (SIT) were used to investigate the anxiolytic-like activity while forced swim test (FST) and tail suspension test (TST) were employed for the antidepressant effect. Mice were pre-treated orally with purified water (10 mL/kg), bromazepam (1 mg/kg), fluoxetine (20 mg/kg) and (25, 50, 100 and 200 mg/kg). One hour post-treatment, mice were subjected to the various tests.
In HBT, increased the head dips and sectional crossings turnover. increased the number of square crossed and rearings/assisted rearings in OFT. increased the time spent in open arms of EPM. In SIT, increased the frequency/duration of interactions. In FST and TST, decreased the duration of immobility which were reversed by doxazosin (α adrenoceptor antagonist) and ondasentron (5-HT receptor antagonist).
HLDS has anxiolytic- and antidepressant-like activities through modulation of serotoninergic and adrenergic neurotransmissions.
民族植物学调查发现 可用于治疗精神健康异常。本研究旨在探讨 水醇叶提取物（HLDS）在小鼠中的抗抑郁和抗焦虑样活性及其可能的作用机制。
采用洞板试验（HBT）、旷场试验（OFT）、高架十字迷宫试验（EPMT）和社交互动试验（SIT）来研究抗焦虑样活性，而强迫游泳试验（FST）和悬尾试验（TST）则用于评估抗抑郁作用。小鼠经口预先给予纯化水（10 mL/kg）、溴西泮（1 mg/kg）、氟西汀（20 mg/kg）和 （25、50、100 和 200 mg/kg）。给药 1 小时后，对小鼠进行各种测试。
在 HBT 中， 增加了头浸次数和截面交叉次数。 在 OFT 中增加了方格交叉次数和后肢站立/辅助后肢站立次数。 在 EPM 中增加了进入开放臂的时间。在 SIT 中， 增加了互动的频率/持续时间。在 FST 和 TST 中， 减少了不动时间，这一作用可被多沙唑嗪（α 肾上腺素受体拮抗剂）和昂丹司琼（5-HT 受体拮抗剂）逆转。
HLDS 通过调节 5-羟色胺能和肾上腺素能神经传递，具有抗焦虑和抗抑郁样活性。
