Department of Respiratory Medicine in Zhejiang Hospital Affiliated To Medical School of Zhejiang University, 1229 Gudun Road, Xihu District, Hangzhou, 310012, Zhejiang, China.
Curr Microbiol. 2022 Feb 26;79(4):116. doi: 10.1007/s00284-022-02811-x.
Intestinal microbiota disorders can aggravate pulmonary inflammation during acute lung injury (ALI). As a traditional Chinese herb, Rhubarb can regulated the gut microbiota. Therefore, this study was conducted to test the hypothesis that rhubarb alleviates gut microbiota dysbiosis and inflammation. Feces were collected from patients with ALI to detect the gut microbiota using 16S rDNA sequencing. Subsequently, a mouse model of ALI was established using lipopolysaccharide to investigate changes in the gut microbiota, the peripheral blood was attained for detecting the Th17/Treg cell ratio and the serum level of HDAC6 and HDAC9, and the effect of rhubarb treatment on the gut microbiota and Th17/Treg ratio were also evaluated. The results indicated that both the Firmicutes phylum decreased and the Bacteroidetes phylum increased were identified in patients with ALI, which induced the alternation of histone metabolites. The mice models also showed a similar imbalance in the Firmicutes/Bacteroidetes ratio at phylum of level. Rhubarb treatment alleviated the damaged lung tissue, accelerated Alistipes, Clostridium, and Lactobacillus proliferation at the level of genus, increased the level of HDAC6 in both the mice lung tissue and serum, and markedly reduced the Treg cells and increased the Th17 cells in the spleen tissue. The study suggested that both patients and mouse models with ALI presented gut microbiota dysbiosis, and lead to a Th17/Treg cell imbalance in ALI mouse. Rhubarb promoted Alistipes, Clostridium, and Lactobacillus proliferation, increased the HDAC6 concentration, restored the Th17/Treg cell balance, and protected against ALI.
肠道微生物失调可加重急性肺损伤（ALI）时的肺部炎症。大黄作为一种传统中药，可调节肠道微生物群。因此，本研究旨在验证大黄缓解肠道微生物失调和炎症的假说。收集 ALI 患者的粪便，采用 16S rDNA 测序检测肠道微生物群。随后，采用脂多糖建立 ALI 小鼠模型，检测肠道微生物群的变化，采集外周血检测 Th17/Treg 细胞比值及血清中 HDAC6 和 HDAC9 水平，并评价大黄对肠道微生物群和 Th17/Treg 比值的影响。结果表明，ALI 患者厚壁菌门减少，拟杆菌门增加，诱导组蛋白代谢物的改变。在小鼠模型中，厚壁菌门/拟杆菌门比值也存在类似的失衡。大黄治疗可缓解受损的肺组织，加速属水平上的 Alistipes、Clostridium 和 Lactobacillus 的增殖，增加肺组织和血清中 HDAC6 的水平，显著减少脾脏组织中的 Treg 细胞，增加 Th17 细胞。本研究表明，ALI 患者和小鼠模型均存在肠道微生物失调，并导致 ALI 小鼠 Th17/Treg 细胞失衡。大黄促进 Alistipes、Clostridium 和 Lactobacillus 的增殖，增加 HDAC6 浓度，恢复 Th17/Treg 细胞平衡，从而起到保护 ALI 的作用。
