Wang Tingxiang, Su Wanting, Li Li, Wu Haiyan, Huang He, Li Zhijun
Department of Oncology, Zhejiang Hospital Affiliated with the Medical SChool of Zhejiang University, 1229 Gudun Road, Xihu District, Hangzhou, Zhejiang 310012, China.
Zhejiang Chinese Medical University, 348 Binwen Road, Binjiang District, Hangzhou, Zhejiang 310000, China.
Heliyon. 2024 Apr 26;10(9):e30380. doi: 10.1016/j.heliyon.2024.e30380. eCollection 2024 May 15.
To explore the abundance and diversity of the gut microbiota in patients with lung cancer accompanied by chronic obstructive pulmonary disease (LC-COPD).
The study cohort comprised 15 patients with LC-COPD, 49 patients with lung cancer, and 18 healthy control individuals. ELISA was used to detect inflammatory factors in venous blood. 16S rDNA sequencing was performed to determine the abundance and diversity of the gut microbiota. Gas chromatography-mass spectrometry was used to determine the concentration of short-chain fatty acids (SCFAs) in feces samples.
The α-diversity index indicated that the richness and diversity of the gut microbiota were lower in patients with LC-COPD compared with patients with lung cancer and controls. Principal component analysis revealed significant differences among the three groups ( < 0.05). The linear discriminant analysis effect size algorithm indicated that the o_, g_, f_ s_, c_ g_, s_, and s_ species were prevalent in patients with LC-COPD, while the g_ and g_ species were prevalent in patients with lung cancer. Furthermore, the concentrations of the SCFAs butyric acid, isobutyric acid, isovaleric acid, and valeric acid tended to be lower in patients with LC-COPD compared with patients with lung cancer and healthy controls, although these intergroup differences were not significant ( > 0.05). Patients with lung cancer had the lowest serum concentration of tumor necrosis factor (TNF)-a. There were no intergroup differences in the concentrations of other inflammatory factors.
The present study indicated that the abundance and structure of the gut microbiota is altered, and the concentrations of SCFAs may be decreased in patients with LC-COPD. In addition, patients with lung cancer had the lowest serum concentration of TNF-a.
探讨肺癌合并慢性阻塞性肺疾病（LC-COPD）患者肠道微生物群的丰度和多样性。
研究队列包括15例LC-COPD患者、49例肺癌患者和18名健康对照个体。采用酶联免疫吸附测定（ELISA）法检测静脉血中的炎症因子。进行16S核糖体DNA（rDNA）测序以确定肠道微生物群的丰度和多样性。采用气相色谱-质谱联用技术测定粪便样本中短链脂肪酸（SCFA）的浓度。
α多样性指数表明，与肺癌患者和对照组相比，LC-COPD患者肠道微生物群的丰富度和多样性较低。主成分分析显示三组之间存在显著差异（P<0.05）。线性判别分析效应大小算法表明，o_、g_、f_、s_、c_、g_、s_和s_物种在LC-COPD患者中普遍存在，而g_和g_物种在肺癌患者中普遍存在。此外，与肺癌患者和健康对照相比，LC-COPD患者中丁酸、异丁酸、异戊酸和戊酸等SCFA的浓度往往较低，尽管这些组间差异不显著（P>0.05）。肺癌患者血清肿瘤坏死因子（TNF）-α浓度最低。其他炎症因子浓度在组间无差异。
本研究表明，LC-COPD患者肠道微生物群的丰度和结构发生改变，SCFA浓度可能降低。此外，肺癌患者血清TNF-α浓度最低。
