荧光显微镜：生物学家实用指南。

Fluorescence Microscopy: A Field Guide for Biologists.

机构信息

Department of Physiology and Pharmacology, Live Cell Imaging Laboratory, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada.

出版信息

Methods Mol Biol. 2022;2440:3-39. doi: 10.1007/978-1-0716-2051-9_1.

DOI:10.1007/978-1-0716-2051-9_1

PMID:35218530

Abstract

Optical microscopy is a tool for observing objects, and features within objects, that are not visible to the unaided eye. In the life sciences, fluorescence microscopy has been widely adopted because it allows us to selectively observe molecules, organelles, and cells at multiple levels of organization. Fluorescence microscopy encompasses numerous techniques and applications that share a specialized technical language and concepts that can create barriers for researchers who are new to this area. Our goal is to meet the needs of researchers new to fluorescence microscopy, by introducing the essential concepts and mindset required to navigate and apply this powerful technology to the laboratory.

摘要

光学显微镜是一种用于观察肉眼无法看到的物体和物体内部特征的工具。在生命科学领域，荧光显微镜已被广泛采用，因为它可以让我们选择性地观察分子、细胞器和细胞在多个组织层次上的状态。荧光显微镜涵盖了许多技术和应用，这些技术和应用都有其专门的技术语言和概念，这可能会给刚接触这个领域的研究人员带来障碍。我们的目标是满足刚接触荧光显微镜的研究人员的需求，介绍必要的概念和思维模式，以帮助他们掌握并在实验室中应用这项强大的技术。