抗体验证的十条基本规则。
Ten Basic Rules of Antibody Validation.
作者信息
Weller Michael G
机构信息
Division 1.5 Protein Analysis, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany.
出版信息
Anal Chem Insights. 2018 Feb 8;13:1177390118757462. doi: 10.1177/1177390118757462. eCollection 2018.
Abstract
The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria.
摘要
研究用抗体的质量问题已经存在数十年了。尽管已经发表了几篇论文来改善这种情况，但其影响似乎有限。本出版物致力于以一种方式简化验证标准的描述，使偶尔使用抗体的用户能够评估免疫化学试剂的验证水平。还提供了一份简单的1页清单，用于这些标准的实际应用。
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