MRC Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK.
Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China.
Methods Mol Biol. 2022;2440:305-326. doi: 10.1007/978-1-0716-2051-9_18.
The structural organization of macromolecules and their association in assemblies and organelles is key to understand cellular function. Super-resolution fluorescence microscopy has expanded our toolbox for examining such nanometer-scale cellular structures, by enabling positional mapping of proteins in situ. Here, we detail the workflow to build nanometer-scale maps focusing on two complementary super-resolution modalities: structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM).
大分子的结构组织及其在组装体和细胞器中的关联是理解细胞功能的关键。超分辨率荧光显微镜通过在原位对蛋白质进行定位映射，扩展了我们研究这种纳米级细胞结构的工具包。在这里，我们详细介绍了构建纳米级图谱的工作流程，重点介绍了两种互补的超分辨率模式：结构光照明显微镜（SIM）和随机光学重建显微镜（STORM）。
