Kah O, Breton B, Dulka J G, Núnez-Rodríguez J, Peter R E, Corrigan A, Rivier J E, Vale W W
Cell Tissue Res. 1986;244(2):327-37. doi: 10.1007/BF00219208.
The organization of Gn-RH systems in the brain of teleosts has been investigated previously by immunohistochemistry using antibodies against the mammalian decapeptide which differs from the teleostean factor. Here, we report the distribution of immunoreactive Gn-RH in the brain of goldfish using antibodies against synthetic teleost peptide. Immunoreactive structures are found along a column extending from the rostral olfactory bulbs to the pituitary stalk. Cell bodies are observed within the olfactory nerves and bulbs, along the ventromedial telencephalon, the ventrolateral preoptic area and the latero-basal hypothalamus. Large perikarya are detected in the dorsal midbrain tegmentum, immediately caudal to the posterior commissure. A prominent pathway was traced from the cells located in the olfactory nerves through the medial olfactory tract and along all the perikarya described above to the pituitary stalk. In the pituitary, projections are restricted to the proximal pars distalis. A second immunoreactive pathway ascends more dorsally in the telencephalon and arches to the periventricular regions of the diencephalon. Part of this pathway forms a periventricular network in the dorsal and posterior hypothalamus, whereas other projections continue caudally to the medulla oblongata and the spinal cord. Lesions of the ventral preoptic area demonstrate that most of the fibers detected in the pituitary originate from the preoptic region.
先前已通过免疫组织化学方法，使用针对与硬骨鱼类因子不同的哺乳动物十肽的抗体，对硬骨鱼类大脑中的促性腺激素释放激素（Gn-RH）系统进行了研究。在此，我们报告使用针对合成硬骨鱼类肽的抗体，在金鱼大脑中免疫反应性Gn-RH的分布情况。免疫反应性结构沿着从吻端嗅球延伸至垂体柄的柱状区域分布。在嗅神经和嗅球内、腹内侧端脑、腹外侧视前区以及外侧基底下丘脑内均观察到细胞体。在紧靠后连合后方的背侧中脑被盖中检测到大型核周体。一条显著的通路从位于嗅神经中的细胞出发，经内侧嗅束并沿着上述所有核周体延伸至垂体柄。在垂体中，投射仅限于远侧部近端。第二条免疫反应性通路在端脑背侧上升并弯向间脑的室周区域。该通路的一部分在背侧和后部下丘脑形成室周网络，而其他投射继续向后延伸至延髓和脊髓。腹侧视前区损伤表明，在垂体中检测到的大多数纤维起源于视前区。
