Belasco J G, Nilsson G, von Gabain A, Cohen S N
Cell. 1986 Jul 18;46(2):245-51. doi: 10.1016/0092-8674(86)90741-5.
To map the structural features responsible for the 5-fold difference in stability of the E. coli ompA and bla gene transcripts, we have constructed gene fusions that encode chimeric ompA/bla transcripts and a deletion that eliminates a large internal segment of bla mRNA. Shortening of bla transcripts by internal deletion or replacement of the 3' end with the corresponding segment of the ompA transcript had little effect on bla mRNA stability. However, fusion of a 5'-terminal 147 nucleotide segment of the ompA message 5' to full-length or truncated bla transcripts increased the half-life of the bla segments 3- to 5-fold. These and other findings indicate that E. coli transcripts contain discrete structural determinants of stability and instability that can influence the decay rate of linked mRNA segments derived from other genes.
为了绘制出导致大肠杆菌ompA和bla基因转录本稳定性相差5倍的结构特征图谱，我们构建了编码嵌合ompA/bla转录本的基因融合体以及一个删除bla mRNA大片内部片段的缺失突变体。通过内部缺失或用ompA转录本的相应片段替换3'末端来缩短bla转录本，对bla mRNA稳定性影响不大。然而，将ompA信使RNA 5'端的147个核苷酸片段与全长或截短的bla转录本融合，可使bla片段的半衰期提高3至5倍。这些及其他发现表明，大肠杆菌转录本包含稳定性和不稳定性的离散结构决定因素，这些因素可影响源自其他基因的相连mRNA片段的降解速率。
